Science in China Series B: Chemistry

, Volume 52, Issue 10, pp 1576–1582

Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

  • Hao Yue
  • Wan Chan
  • KongJie Yu
  • Lin Guo
  • Ning Liu
  • HuWei Liu
  • ZongWei Cai
Article

DOI: 10.1007/s11426-009-0233-6

Cite this article as:
Yue, H., Chan, W., Yu, K. et al. Sci. China Ser. B-Chem. (2009) 52: 1576. doi:10.1007/s11426-009-0233-6
  • 67 Views

Abstract

Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

Keywords

aristolochic acidDNA adductsquantitative analysisshort review

Copyright information

© Science in China Press and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Hao Yue
    • 1
    • 2
  • Wan Chan
    • 1
  • KongJie Yu
    • 3
  • Lin Guo
    • 1
  • Ning Liu
    • 1
    • 2
  • HuWei Liu
    • 4
  • ZongWei Cai
    • 1
  1. 1.Department of ChemistryHong Kong Baptist UniversityHong Kong SARChina
  2. 2.Changchun University of Chinese MedicineChangchunChina
  3. 3.Fujian Entry-Exit Inspection and Quarantine BureauFuzhouChina
  4. 4.Institute of Analytical Chemistry, College of Chemistry and Molecular EngineeringPeking UniversityBeijingChina