Journal of Natural Medicines

, Volume 67, Issue 3, pp 662–671

Polypharmacy (herbal and synthetic drug combination): a novel approach in the treatment of type-2 diabetes and its complications in rats


  • Rupinder Kaur
    • Siddhartha Institute of Pharmacy
    • Pacific University
    • Siddhartha Institute of Pharmacy
  • Imran Kazmi
    • Siddhartha Institute of Pharmacy
  • Iqbal Ahamd
    • Department of Pharmaceutics, Faculty of PharmacyJamia Hamdard
  • Zubair Ahmed
    • Sebai Institute of Health Sciences
  • Babar Ali
    • Department of Pharmacognosy, Faculty of PharmacyJamia Hamdard
  • Sayeed Ahmad
    • Department of Pharmacognosy, Faculty of PharmacyJamia Hamdard
    • Siddhartha Institute of Pharmacy

DOI: 10.1007/s11418-012-0720-5

Cite this article as:
Kaur, R., Afzal, M., Kazmi, I. et al. J Nat Med (2013) 67: 662. doi:10.1007/s11418-012-0720-5


The aim of present study was to evaluate the effect of aqueous leaf extract of Annona squamosa with Glipizide in a high fat diet and streptozocin-induced type-2 diabetes. Nine groups (n = 6) of male Sprague-Dawley rats were used for the study, with. Basal blood glucose, urine volume, and body weights were measured and the rate were kept on a high fat diet. After 15 days, streptozocin in sub-diabetic dose (35 mg/kg) was administered to the animals to induce diabetes. With 1 week of consistent hyperglycemia, treatment was initiated. Aqueous extract of Annona squamosa was administered orally at 350 mg/kg body weight alone and in combination with reduced and reducing dose combinations of Glipizide. Blood glucose, body weight, urine volume were measured every 10th day. The elevated blood-glucose level in diabetic rats was controlled better with combination therapy compared with the synthetic drug alone or the herbal stand-alone drug. All the results were statistically significant (P < 0.001). A combination of Annona squamosa along with Glipizide may be helpful in dose reduction of Glipizide up to 50 %, reducing the risk of the onset of insulin therapy.


Annona squamosaGlipizideCombination therapyBiochemical parameter

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© The Japanese Society of Pharmacognosy and Springer Japan 2012