Journal of Natural Medicines

, Volume 66, Issue 1, pp 55–61

Patchouli alcohol: in vitro direct anti-influenza virus sesquiterpene in Pogostemon cablin Benth.

Authors

    • Department of Drug Discovery Sciences, Kitasato Institute for Life SciencesKitasato University
    • Graduate School of Infection Control SciencesKitasato University
    • Department of Basic Research, Oriental Medicine Research CenterKitasato University
  • Chikara Ichino
    • Department of Drug Discovery Sciences, Kitasato Institute for Life SciencesKitasato University
  • Yuka Kawamura
    • Graduate School of Infection Control SciencesKitasato University
  • Takayuki Nagai
    • Department of Drug Discovery Sciences, Kitasato Institute for Life SciencesKitasato University
    • Graduate School of Infection Control SciencesKitasato University
    • Department of Basic Research, Oriental Medicine Research CenterKitasato University
  • Noriko Sato
    • Department of Central Analytical Laboratory, School of PharmacyKitasato University
    • Department of Drug Discovery Sciences, Kitasato Institute for Life SciencesKitasato University
    • Graduate School of Infection Control SciencesKitasato University
    • Department of Basic Research, Oriental Medicine Research CenterKitasato University
Original Paper

DOI: 10.1007/s11418-011-0550-x

Cite this article as:
Kiyohara, H., Ichino, C., Kawamura, Y. et al. J Nat Med (2012) 66: 55. doi:10.1007/s11418-011-0550-x

Abstract

During the screening of anti-influenza virus substances from traditional herbal medicines, the methanol extract from the leaves of Pogostemon cablin Benth. showed potent in vitro antiviral activity (99.8% inhibition at a concentration of 10 μg/mL) against influenza virus A/PR/8/34 (H1N1). The anti-influenza virus principle was isolated from the hexane-soluble fraction, through solvent fractionation, repeated silica gel column chromatography, and reversed-phase HPLC. The major active principle was a volatile substance that was identified as a sesquiterpene, patchouli alcohol (1), on the basis of its spectral analyses. When anti-influenza virus activity against A/PR/8/34 was evaluated by the plaque forming assay, patchouli alcohol reduced the number of plaques by 75% at 2 μg/mL and 89% at 10 μg/mL. Patchouli alcohol showed dose-dependent anti-influenza virus activity, and its IC50 value was estimated to be 2.635 μM. Although 11 different sesquiterpenes were tested for antiviral activity against influenza virus A/PR/8/34, no or negligible activity was observed except for patchouli alcohol. Patchouli alcohol did not show anti-influenza virus activity against A/Guizhou/54/89 (H3N2), but showed weak activity against B/Ibaraki/2/85 (IC50 = 40.82 μM). Patchouli alcohol did not show inhibitory activity against influenza virus neuraminidase.

Keywords

Pogostemon cablin Benth.Lamiaceae (Labiatae)Patchouli alcoholInfluenza virus

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2011