Skip to main content

Advertisement

SpringerLink
Log in

Evaluation of anti-inflammatory and antioxidant activities of Peltigera rufescens lichen species in acute and chronic inflammation models

  • Original Paper
  • Published:
Journal of Natural Medicines Aims and scope Submit manuscript

Abstract

The anti-inflammatory effects of the methanol extract of the lichen species Peltigera rufescens (Weis.) Humb (MEPR) (Peltigeraceae) on acute (carrageenan-induced) and chronic (cotton pellet granule) phases of inflammation were investigated. The MEPR was capable of reducing carrageenan-induced inflammation and showed a potent antiproliferative effect (63.5%) in the chronic inflammation model. Inflammation is related to neutrophil infiltration and the production of neutrophil-derived mediators and free radicals. The MEPR reduced the myeloperoxidase and inducible nitric oxide synthase (NOS) activities, which were increased by carrageenan injection. Carrageenan injection also increased the lipid peroxidation (LPO) as compared with untreated paw tissues. The administration of MEPR, diclofenac, and indomethacin reduced the LPO in paw tissues through amelioration of the antioxidant defense systems. Neutrophil infiltration and neutrophil-derived free radicals in tissues therefore appeared to play an important role in the inflammation process induced by carrageenan. The anti-inflammatory effect of MEPR could be attributed to its reducing effect on the neutrophil-derived free radicals and its ameliorating effect on the antioxidant defense systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Di Rosa M, Willoughby DA (1971) Screens for anti-inflammatory drugs. J Pharm Pharmacol 23:297–305

    CAS  PubMed  Google Scholar 

  2. Syahida A, Israf DA, Lajis NH, Khozirah S, Habsah M, Permana D, Norhadiani I (2006) Effect of compounds isolated from natural products on IFN-c/LPS-induced nitric oxide production in RAW 264.7 macrophages. Pharm Biol 44:50–59

    Article  CAS  Google Scholar 

  3. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem-Biol Interact 160:1–40

    Article  CAS  PubMed  Google Scholar 

  4. FitzGerald G, Patrono C (2001) The coxibs, selective inhibition of cyclooxygenase-2. New Engl J Med 345:433–442

    Article  CAS  PubMed  Google Scholar 

  5. Emerit J, Edeas A, Bricaire F (2004) Neurodegenerative diseases and oxidative stress. Biomed Pharmacother 58:39–46

    Article  CAS  PubMed  Google Scholar 

  6. Conner EM, Grisham MB (1996) Inflammation, free radicals and antioxidants. Nutrition 12:274–277

    Article  CAS  PubMed  Google Scholar 

  7. Davies KJA (2000) Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life 50:279–289

    Article  CAS  PubMed  Google Scholar 

  8. Huneck S, Yoshimura I (1996) Identification of lichen substances. Springer, Berlin, pp 1–493

    Google Scholar 

  9. Odabasoglu F, Aslan A, Cakir A, Suleyman H, Karagoz Y, Bayir Y, Halici M (2005) Antioxidant activity, reducing power and total phenolic content of some lichen species. Fitoterapia 76:216–219

    Article  CAS  PubMed  Google Scholar 

  10. Suleyman H, Odabasoglu F, Aslan A, Cakir A, Karagoz Y, Gocer F, Halici M, Bayir Y (2003) Antiinflammatory and antiulcer effects of aqueous extract of Lobaria pulmonaria. Phytomedicine 10:552–557

    Article  CAS  PubMed  Google Scholar 

  11. Ohkawa H, Ohishi H, Yagi K (1979) Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  CAS  PubMed  Google Scholar 

  12. Sun Y, Larry WO, Ying L (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500

    CAS  PubMed  Google Scholar 

  13. Aebi H (1984) Catalase. Method Enzymol 105:121–126

    Article  CAS  Google Scholar 

  14. Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryls groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205

    Article  CAS  PubMed  Google Scholar 

  15. Calberg I, Mannervik B (1985) Glutathione reductase. Method Enzymol 113:484–490

    Article  Google Scholar 

  16. Habig WH, Jakoby WB (1981) Assays for differentiation glutathione-S-transferase. Method Enzymol 77:398–405

    Article  CAS  Google Scholar 

  17. Lawrence RA, Burk RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958

    Article  CAS  PubMed  Google Scholar 

  18. Bradley PP, Priebat DA, Christensen RD, Rothstein G (1982) Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 78:206–209

    Article  CAS  PubMed  Google Scholar 

  19. Knowles RG, Merrett M, Salter M, Moncada S (1990) Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. Biochem J 270:833–836

    CAS  PubMed  Google Scholar 

  20. Shacter E, Lopez RL, Pati S (1991) Inhibition of the myeloperoxidase–H2O2–Cl− system of neutrophils by indomethacin and other non-steroidal anti-inflammatory drugs. Biochem Pharmacol 41:975–984

    Article  CAS  PubMed  Google Scholar 

  21. Pabuccuoglu A, Konyalioglu S, Bas M, Maral GE (2003) The in vitro effects of Hypericum species on human leukocyte myeloperoxidase activity. J Ethnopharmacol 87:89–92

    Article  PubMed  Google Scholar 

  22. Paino IMM, Ximenes VF, da Fonseca LM, Kanegae MPP, Khalil NM, Brunetti IL (2005) Effect of therapeutics plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils. Braz J Med Biol Res 38:543–551

    Article  CAS  PubMed  Google Scholar 

  23. Lee CS, Jang YY, Song JS, Song JH, Han ES (2002) Ambroxol inhibits peroxynitrite-induced damage of a 1-antiproteinase and free radical production in activated phagocytic cells. Pharmacol Toxicol 91:140–149

    Article  CAS  PubMed  Google Scholar 

  24. Halici Z, Dengiz GO, Odabasoglu F, Suleyman H, Cadirci E, Halici M (2007) Amiadorane has anti-inflammatory and anti-oxidative properties: an experimental study in rats with carrageenan-induced paw edema. Eur J Pharmacol 566:215–221

    Article  CAS  PubMed  Google Scholar 

  25. Nussler AK, Billiar TR (1993) Inflammation, immunoregulation, and inducible nitric oxide synthase. J Leucocyte Biol 54:171–178

    CAS  Google Scholar 

  26. Salvemini D, Wang ZQ, Wyatt DM, Bourdon MH, Marino PT, Currie MG (1996) Nitric oxide: a key mediator in the early and late phase of carrageenan-induced rat paw inflammation. Brit J Pharmacol 118:829–838

    CAS  Google Scholar 

  27. Wang L, Tu YC, Lian TW, Hung JT, Yen JH, Wu MJ (2006) Distinctive antioxidant and anti-inflammatory effects of flavonoids. J Agric Food Chem 54:9798–9804

    Article  CAS  PubMed  Google Scholar 

  28. Wu Y, Zhou C, Song L, Li X, Shi S, Mo J, Chen H, Bai H, Wu X, Zhao J, Zhang R, Hao X, Sun H, Zhao Y (2006) Effect of total phenolics from Laggera alata on acute and chronic inflammation models. J Ethnopharmacol 108:243–250

    Article  CAS  PubMed  Google Scholar 

  29. Wu Y, Zhou C, Li X, Song L, Wu X, Lin W, Chen H, Bai H, Zhao J, Zhang R, Sun H, Zhao Y (2006) Evaluation of anti-inflammatory activity of the total flavonoids of Laggera pterodonta on acute and chronic inflammation models. Phytother Res 20:585–590

    Article  CAS  PubMed  Google Scholar 

  30. Wang ZQ, Porreca F, Cuzzocrea S, Galen K, Lightfoot R, Masini E, Muscoli C, Mollace V, Ndengale M, Ischiropoulos H, Salvemini D (2004) A newly identified role for superoxide in inflammatory pain. J Pharm Exp Ther 309:869–878

    Article  CAS  Google Scholar 

  31. Cadenas E (1989) Biochemistry of oxygen toxicity. Ann Rev Biochem 58:79–110

    Article  CAS  PubMed  Google Scholar 

  32. Govindarajan R, Vijayakumar M, Rao Ch V, Shirwaikar A, Kumar S, Rawat AKS, Pushpangadan P (2007) Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats. Phytother Res 21:975–979

    Article  CAS  PubMed  Google Scholar 

  33. Chen G, Kamal M, Hannon R, Warner TD (1998) Regulation of cyclo-oxygenase gene expression in rat smooth muscle cells by catalase. Biochem Pharmacol 55:1621–1631

    Article  CAS  PubMed  Google Scholar 

Download references

Conflict of interest statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey

    Sevil Tanas & Fehmi Odabasoglu

  2. Department of Pharmacology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey

    Zekai Halici & Halis Suleyman

  3. Department of Chemistry, Faculty of Science and Art, Kilis 7 Aralık University, 79100, Kilis, Turkey

    Ahmet Cakir

  4. Department of Orthopaedics Traumatology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey

    Hayati Aygun

  5. Department of Biology, K. K. Education Faculty, Ataturk University, 25240, Erzurum, Turkey

    Ali Aslan

Authors
  1. Sevil Tanas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fehmi Odabasoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zekai Halici
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ahmet Cakir
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hayati Aygun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ali Aslan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Halis Suleyman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ahmet Cakir.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanas, S., Odabasoglu, F., Halici, Z. et al. Evaluation of anti-inflammatory and antioxidant activities of Peltigera rufescens lichen species in acute and chronic inflammation models. J Nat Med 64, 42–49 (2010). https://doi.org/10.1007/s11418-009-0367-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11418-009-0367-z

Keywords

Search

Navigation