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Crambescidin 800, a pentacyclic guanidine alkaloid, protects a mouse hippocampal cell line against glutamate-induced oxidative stress

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Abstract

Oxidative stress is implicated in the pathogenesis of neuronal degenerative diseases. In our search for protective substances against glutamate-induced oxidative stress using mouse hippocampal cell line HT22, we have isolated crambescidin 800, a pentacyclic guanidine alkaloid, from the Indonesian marine sponge, Monanchora ungiculata. Crambescidin 800 (1) protected HT22 cells against glutamate-induced oxidative toxicity at 0.06 μM (EC50) concentration. Crambescidin 800 (1) also protected HT22 and neuroblastoma cells from the oxidative stress induced by a hypoxic condition or nitric oxide (NO). Crambescidin 800 (1) showed no effect on the depletion of intracellular glutathione (GSH) of HT22 cells induced by glutamate, and production of reactive oxygen species (ROS) and activation of mitogen-activated protein kinase (MAPK) were inhibited only by the high concentration (1 μM) of 1.

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Acknowledgments

The authors are grateful to Prof. R. W. M. Soest, Zoologisch Museum, University of Amsterdam, for identification of the sponge and also grateful to Dr. Kumiko Ishige, College of Pharmacy, Nihon University, and Dr. Takashi Kudo, Graduate School of Medicine, Osaka University, for the gift of HT22 and SH-SY5Y cells, respectively. This study was financially supported by Grant-in-Aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka, 565-0871, Japan

    Hideaki Suna, Shunji Aoki & Motomasa Kobayashi

  2. Department of Chemistry, Faculty of Science, Lampung University, Jl. Prof. Dr. Sumantri Brodjonegoro No. 1, Bandar Lampung, 35145, Indonesia

    Andi Setiawan

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  1. Hideaki Suna
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  2. Shunji Aoki
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  4. Motomasa Kobayashi
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Correspondence to Motomasa Kobayashi.

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Suna, H., Aoki, S., Setiawan, A. et al. Crambescidin 800, a pentacyclic guanidine alkaloid, protects a mouse hippocampal cell line against glutamate-induced oxidative stress. J Nat Med 61, 288–295 (2007). https://doi.org/10.1007/s11418-007-0148-5

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  • DOI: https://doi.org/10.1007/s11418-007-0148-5

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