Journal of Biomedical Science

, 15:771

Enhancement of ectopic bone formation by bone morphogenetic protein-2 delivery using heparin-conjugated PLGA nanoparticles with transplantation of bone marrow-derived mesenchymal stem cells

Authors

  • Sung Eun Kim
    • Department of Oral Biology and Institute of Oral Biology, School of DentistryKyung Hee University
  • Oju Jeon
    • Weldon School of Biomedical Engineering and Department of PharmaceuticsPurdue University
  • Jung Bok Lee
    • Department of Oral Biology and Institute of Oral Biology, School of DentistryKyung Hee University
  • Min Soo Bae
    • Department of Oral Biology and Institute of Oral Biology, School of DentistryKyung Hee University
  • Heoung-Jae Chun
    • Department of Mechanical Engineering, School of EngineeringYonsei University
  • Seong-Hwan Moon
    • Department of Orthopaedic Surgery, School of MedicineYonsei University
    • Department of Oral Biology and Institute of Oral Biology, School of DentistryKyung Hee University
Original Paper

DOI: 10.1007/s11373-008-9277-4

Cite this article as:
Kim, S.E., Jeon, O., Lee, J.B. et al. J Biomed Sci (2008) 15: 771. doi:10.1007/s11373-008-9277-4

Abstract

This study was performed to determine if a combination of previously undifferentiated bone marrow-derived mesenchymal stem cells (BMMSCs) and exogenous bone morphogenetic protein-2 (BMP-2) delivered via heparin-conjugated PLGA nanoparticles (HCPNs) would extensively regenerate bone in vivo. In vitro testing found that the HCPNs were able to release BMP-2 over a 2-week period. Human BMMSCs cultured in medium containing BMP-2-loaded HCPNs for 2 weeks differentiated toward osteogenic cells expressing alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) mRNA, while cells without BMP-2 expressed only ALP. In vivo testing found that undifferentiated BMMSCs with BMP-2-loaded HCPNs induce far more extensive bone formation than either implantation of BMP-2-loaded HCPNs or osteogenically differentiated BMMSCs. This study demonstrates the feasibility of extensive in vivo bone regeneration by transplantation of undifferentiated BMMSCs and BMP-2 delivery via HCPNs.

Keywords

Bone marrow-derived mesenchymal stem cellBone morphogenetic protein-2Bone regenerationHeparin-conjugated PLGA nanoparticles

Copyright information

© National Science Council Taipei 2008