Journal of Biomedical Science

, Volume 14, Issue 6, pp 777–782

Differences in osteoblast miRNA induced by cell binding domain of collagen and silicate-based synthetic bone

  • Annalisa Palmieri
  • Furio Pezzetti
  • Giorgio Brunelli
  • Ilaria Zollino
  • Luca Scapoli
  • Marcella Martinelli
  • Marzia Arlotti
  • Francesco Carinci
Article

DOI: 10.1007/s11373-007-9193-z

Cite this article as:
Palmieri, A., Pezzetti, F., Brunelli, G. et al. J Biomed Sci (2007) 14: 777. doi:10.1007/s11373-007-9193-z

Summary

PerioGlas (PG) is an silicate-based (i.e. anorganic) material used for grafting periodontal osseous defects since the ninety whereas P-15 is an analog of the cell binding domain of collagen (i.e. organic material) that is successfully used in clinical trial to promote bone formation. However, how PG (i.e anorganic material) and P-15 (i.e. collagen) differentially alter osteoblast activity to promote bone formation is unknown. We therefore attempted to get more insight by using microRNA microarray techniques to investigate the translation process in osteoblasts differentially exposed to PG and P-15. We identified 3 up-regulated miRNA (i.e. mir-30b, mir-26a, mir-92) and 8 down-regulated miRNA (i.e. mir-337, mir-377, mir-25, mir-200b, mir-129, mir-373, mir-133b, mir-489). The data reported are, to our knowledge, the first study on translation regulation in osteoblatsts differentially exposed to cell binding domain of collagen and to silicate-based material. Both enhance the translation of several miRNA belonging to osteogenetic genes, but P-15 acts preferentially on homeobox genes.

Keywords

alloplastic materialallograftmiRNAmicroarraygene expressiongene profiling

Copyright information

© National Science Council Taipei 2007

Authors and Affiliations

  • Annalisa Palmieri
    • 1
    • 2
  • Furio Pezzetti
    • 1
    • 2
  • Giorgio Brunelli
    • 3
  • Ilaria Zollino
    • 3
  • Luca Scapoli
    • 1
    • 2
  • Marcella Martinelli
    • 1
    • 2
  • Marzia Arlotti
    • 1
    • 2
  • Francesco Carinci
    • 3
  1. 1.Institute of HistologyUniversity of BolognaBolognaItaly
  2. 2.Center of Molecular Genetics, CARISBO FoundationUniversity of BolognaBolognaItaly
  3. 3.Department of DMCCC, Section of Maxillofacial SurgeryUniversity of FerraraFerraraItaly