Journal of Biomedical Science

, Volume 14, Issue 4, pp 509–515

Transcriptional regulation of human CYP11A1 in gonads and adrenals

Authors

  • Ing-Cherng Guo
    • Department of Veterinary Medicine, College of Bio-Resources and AgricultureNational Taiwan University
  • Meng-Chun Shih
    • Institute of Molecular Biology, Academia Sinica
    • Institutes of Molecular and Cellular Biology and Department of Life ScienceNational Tsing Hua University
  • Hsin-Chieh Lan
    • Institute of Molecular Biology, Academia Sinica
  • Nai-Chi Hsu
    • Institute of Molecular Biology, Academia Sinica
  • Meng-Chun Hu
    • Graduate Institute of PhysiologyNational Taiwan University College of Medicine
    • Institute of Molecular Biology, Academia Sinica
Article

DOI: 10.1007/s11373-007-9177-z

Cite this article as:
Guo, I., Shih, M., Lan, H. et al. J Biomed Sci (2007) 14: 509. doi:10.1007/s11373-007-9177-z

Summary

The CYP11A1 gene encodes the cholesterol side-chain cleavage enzyme, also termed cytochrome P450scc, which catalyzes the conversion of cholesterol to pregnenolone in the first step of steroid biosynthesis in mitochondria. The adrenal- and gonad-selective, hormonally and developmentally regulated expression of CYP11A1 is principally driven by its 2.3 kb promoter. Multiple trans-acting factors like SF-1, Sp1, AP-2, TReP-132, LBP-1b, LBP-9, AP-1, NF-1, and Ets control CYP11A1 transcription either through DNA-protein interaction with their specific cis-acting elements or through protein-protein interaction between each other, wherein SF-1 plays a central role in adrenals and testes. In addition to binding with its proximal and upstream motifs, SF-1 also physically interacts with TFIIB, CBP/p300, TReP-132, and c-Jun/AP-1 to specifically transmit the regulatory signals of cAMP. Other factors like Sp1 family members, AP-2, and LBP-1b/LBP-9 may be other factors that play a role in CYP11A1 transcription, particularly in placental cells. The TATA sequence could also contribute to tissue-specificity and hormonal regulation of CYP11A1 transcription. This article reviews recent studies focusing on adrenals and gonads.

Keywords

CYP11A1P450scccholesterol side-chain cleavage enzymeSF-1adrenalsteroidogenesissteroidgonadtranscription

Copyright information

© National Science Council Taipei 2007