Journal of Biomedical Science

, Volume 14, Issue 4, pp 509–515

Transcriptional regulation of human CYP11A1 in gonads and adrenals

  • Ing-Cherng Guo
  • Meng-Chun Shih
  • Hsin-Chieh Lan
  • Nai-Chi Hsu
  • Meng-Chun Hu
  • Bon-chu Chung
Article

DOI: 10.1007/s11373-007-9177-z

Cite this article as:
Guo, I., Shih, M., Lan, H. et al. J Biomed Sci (2007) 14: 509. doi:10.1007/s11373-007-9177-z

Summary

The CYP11A1 gene encodes the cholesterol side-chain cleavage enzyme, also termed cytochrome P450scc, which catalyzes the conversion of cholesterol to pregnenolone in the first step of steroid biosynthesis in mitochondria. The adrenal- and gonad-selective, hormonally and developmentally regulated expression of CYP11A1 is principally driven by its 2.3 kb promoter. Multiple trans-acting factors like SF-1, Sp1, AP-2, TReP-132, LBP-1b, LBP-9, AP-1, NF-1, and Ets control CYP11A1 transcription either through DNA-protein interaction with their specific cis-acting elements or through protein-protein interaction between each other, wherein SF-1 plays a central role in adrenals and testes. In addition to binding with its proximal and upstream motifs, SF-1 also physically interacts with TFIIB, CBP/p300, TReP-132, and c-Jun/AP-1 to specifically transmit the regulatory signals of cAMP. Other factors like Sp1 family members, AP-2, and LBP-1b/LBP-9 may be other factors that play a role in CYP11A1 transcription, particularly in placental cells. The TATA sequence could also contribute to tissue-specificity and hormonal regulation of CYP11A1 transcription. This article reviews recent studies focusing on adrenals and gonads.

Keywords

CYP11A1P450scccholesterol side-chain cleavage enzymeSF-1adrenalsteroidogenesissteroidgonadtranscription

Copyright information

© National Science Council Taipei 2007

Authors and Affiliations

  • Ing-Cherng Guo
    • 1
  • Meng-Chun Shih
    • 2
    • 3
  • Hsin-Chieh Lan
    • 2
  • Nai-Chi Hsu
    • 2
  • Meng-Chun Hu
    • 4
  • Bon-chu Chung
    • 2
  1. 1.Department of Veterinary Medicine, College of Bio-Resources and AgricultureNational Taiwan UniversityTaipeiTaiwan
  2. 2.Institute of Molecular Biology, Academia SinicaNankang, TaipeiTaiwan
  3. 3.Institutes of Molecular and Cellular Biology and Department of Life ScienceNational Tsing Hua UniversityHsinchuTaiwan
  4. 4.Graduate Institute of PhysiologyNational Taiwan University College of MedicineTaipeiTaiwan