Journal of Biomedical Science

, Volume 14, Issue 3, pp 313–322

Inhibition of corneal neovascularization with endostatin delivered by adeno-associated viral (AAV) vector in a mouse corneal injury model

Authors

  • Li-Ju Lai
    • Graduate Institute of Clinical Medical Sciences, College of MedicineChang Gung University
    • Department of OphthalmologyChang Gung Memorial Hospital
    • Department of Molecular Genetics and BiochemistryUniversity of Pittsburgh
  • Xiao Xiao
    • Department of Molecular Genetics and BiochemistryUniversity of Pittsburgh
    • Graduate Institute of Clinical Medical Sciences, College of MedicineChang Gung University
    • Department of Microbiology and Immunology, College of MedicineChang Gung University
Article

DOI: 10.1007/s11373-007-9153-7

Cite this article as:
Lai, L., Xiao, X. & Wu, J.H. J Biomed Sci (2007) 14: 313. doi:10.1007/s11373-007-9153-7

Abstract

The use of a recombinant adeno-associated viral (rAAV) vector carrying endostatin gene as an anti-angiogenesis strategy to treat corneal neovascularization in a mouse model was evaluated. Subconjunctival injection of recombinant endostatin-AAV was used to examine the inhibition of corneal neovascularization induced by silver nitrate cauterization in mice. The results showed that gene expression in corneal tissue was observed as early as 4 days after gene transfer and stably lasted for over 8 months with minimal immune reaction. Subconjunctival injection of a high-titer rAAV-endostatin successfully inhibited neovascularization. Immunohistchemistry staining of CD 31 and endostatin showed that the treatment significantly inhibits angiogenesis in cornea. We concluded that the rAAV was capable of directly delivering genes to the ocular surface epithelium by way of subconjunctival injection and was able to deliver sustained, high levels of gene expression in vivo to inhibit angiogenesis.

Keywords

adeno-associated viral (AAV) vectorangiogenesiscorneal neovascularizationendostatin genegene therapyocular surface disease

Copyright information

© National Science Council Taipei 2007