Journal of Biomedical Science

, Volume 14, Issue 1, pp 87–105

Toxicogenomics of A375 human malignant melanoma cells treated with arbutin

  • Sun-Long Cheng
  • Rosa Huang Liu
  • Jin-Nan Sheu
  • Shui-Tein Chen
  • Supachok Sinchaikul
  • Gregory Jiazer Tsay
Article

DOI: 10.1007/s11373-006-9130-6

Cite this article as:
Cheng, SL., Liu, R.H., Sheu, JN. et al. J Biomed Sci (2007) 14: 87. doi:10.1007/s11373-006-9130-6

Summary

Although arbutin is a natural product and widely used as an ingredient in skin care products, its effect on the gene expression level of human skin with malignant melanoma cells is rarely reported. We aim to investigate the genotoxic effect of arbutin on the differential gene expression profiling in A375 human malignant melanoma cells through its effect on tumorigenesis and related side-effect. The DNA microarray analysis provided the differential gene expression pattern of arbutin-treated A375 cells with the significant changes of 324 differentially expressed genes, containing 88 up-regulated genes and 236 down-regulated genes. The gene ontology of differentially expressed genes was classified as belonging to cellular component, molecular function and biological process. In addition, four down-regulated genes of AKT1, CLECSF7, FGFR3, and LRP6 served as candidate genes and correlated to suppress the biological processes in the cell cycle of cancer progression and in the downstream signaling pathways of malignancy of melanocytic tumorigenesis.

Keywords

arbutinA375 cellstoxicogenomicsDNA microarraygene expression

Copyright information

© National Science Council Taipei 2006

Authors and Affiliations

  • Sun-Long Cheng
    • 1
  • Rosa Huang Liu
    • 2
  • Jin-Nan Sheu
    • 1
  • Shui-Tein Chen
    • 3
    • 4
  • Supachok Sinchaikul
    • 3
  • Gregory Jiazer Tsay
    • 5
  1. 1.Institute of MedicineChung Shan Medical UniversityTaichungTaiwan
  2. 2.School of NutritionChung Shan Medical UniversityTaichungTaiwan
  3. 3.Institute of Biological Chemistry and Genomics Research CenterAcademia SinicaTaipeiTaiwan
  4. 4.Institute of Biochemical Sciences, College of Life ScienceNational Taiwan UniversityTaipeiTaiwan
  5. 5.Institute of ImmunologyChung Shan Medical UniversityTaichungTaiwan