Journal of Biomedical Science

, Volume 13, Issue 2, pp 225–232

Thioacetamide induced liver damage in zebrafish embryo as a disease model for steatohepatitis

  • Aseervatham Anusha Amali
  • Ravikumar Deepa Rekha
  • Cliff Ji-Fan Lin
  • Wei-Lun Wang
  • Hong-Yi Gong
  • Gour-Mour Her
  • Jen-Leih Wu
Article

DOI: 10.1007/s11373-005-9055-5

Cite this article as:
Amali, A.A., Rekha, R.D., Lin, C.JF. et al. J Biomed Sci (2006) 13: 225. doi:10.1007/s11373-005-9055-5

Summary

Steatohepatitis has recently been increasing as a cofactor influencing the progression of fibrosis, cirrhosis, adenoma and carcinoma in liver; however, the mechanisms by which it contributes to liver injury remain uncertain. We induced steatohepatitis in zebrafish embryos using thioacetamide (TAA). TUNEL assay revealed significant increasing of apoptosis in liver after 5 days post fertilization and the increasing of apoptosis was observed to be associated with the up-regulation of apoptotic genes such as, bad, bax, P-38a, caspase-3 and 8, and JNK-1. Histological sections by oil red O stain showed the accumulation of fatty droplets which causes the pushing of the nucleus towards one side. Up-regulation of steatosis markers such as, ACC, adiponectin, PTL, CEBP- \(\alpha\) and \(\beta\), SREBP-1 was also observed. Furthermore, the elevation of glutathione peroxidase in TAA treated embryos indicated that TAA induces lipid peroxidation which leads to causes liver damage. Zebrafish has already been considered as a good human disease model and in this context; TAA-treated zebrafish may serve as a good animal model to study the molecular pathogenesis of steatohepatitis. Moreover, non-availability of specific drugs to prevent steatohepatitis, this animal model may serve as a powerful preclinical platform to study the therapeutic strategies and for evaluating chemoprevention strategies for this disease.

Keywords

liver, steatosis, thioacetamide, zebrafish 
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Copyright information

© Springer 2006

Authors and Affiliations

  • Aseervatham Anusha Amali
    • 1
  • Ravikumar Deepa Rekha
    • 1
  • Cliff Ji-Fan Lin
    • 1
    • 2
  • Wei-Lun Wang
    • 1
    • 3
  • Hong-Yi Gong
    • 1
  • Gour-Mour Her
    • 4
  • Jen-Leih Wu
    • 1
  1. 1.Laboratory of Marine Molecular Biology and Biotechnology 301Institute Cellular and Organismic Biology, Academia SinicaTaipeiTaiwan
  2. 2.Graduate Institute of Life SciencesNational Defense Medical CenterTaipeiTaiwan
  3. 3.Institute of Fisheries SciencesNational Taiwan UniversityTaipeiTaiwan
  4. 4.Graduate Institute of BiotechnologyNational Taiwan Ocean UniversityKeelungTaiwan