Journal of Biomedical Science

, Volume 12, Issue 2, pp 297–310

Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy

  • Hua-Ling Chen
  • Chieh-Ju C. Tang
  • Chiung-Ya Chen
  • Tang K. Tang
Article

DOI: 10.1007/s11373-005-0980-0

Cite this article as:
Chen, H., Tang, C.C., Chen, C. et al. J Biomed Sci (2005) 12: 297. doi:10.1007/s11373-005-0980-0

Abstract

Aurora kinases are emerging as key regulators of centrosome function, chromosome segregation and cytokinesis. We previously isolated Aurora-C (Aie1), a third type of Aurora kinase, in a screen for kinases expressed in mouse sperm and eggs. Currently, we know very little about the precise localization and function of Aurora-C. Immunofluorescence analysis of ectopically expressed GFP-Aurora-C has revealed that Aurora-C is a new member of the chromosomal passenger proteins localizing first to the centromeres and then to the central spindles during cytokinesis. In order to study the potential role of Aurora-C, we examined the effects of a kinase-deficient (KD) mutant (AurC-KD) in HeLa Tet-Off cells under tetracycline control. Our results showed that overexpression of AurC-KD causes defects in cell division and induces polyploidy and apoptosis. Interestingly, AurC-KD overexpression also inhibits centromere/kinetochore localization of Aurora-B, Bub1, and BubR1, reduces histone H3 phosphorylation, and disrupts the association of INCENP with Aurora-B. Together, our results showed that Aurora-C is a chromosomal passenger protein, which may serve as a key regulator in cell division.

Keywords

Aurora kinasecell cyclechromosome segregationcytokinesismeiosismitosis

Copyright information

© National Science Council, Taipei 2005

Authors and Affiliations

  • Hua-Ling Chen
    • 1
  • Chieh-Ju C. Tang
    • 1
  • Chiung-Ya Chen
    • 1
  • Tang K. Tang
    • 1
  1. 1.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan, ROC