Regulation of a novel cell differentiation-associated gene, JWA during oxidative damage in K562 and MCF-7 cells Article Received: 01 October 2004 Accepted: 04 November 2004 DOI:
Cite this article as: Zhu, T., Chen, R., Li, AP. et al. J Biomed Sci (2005) 12: 219. doi:10.1007/s11373-004-8186-4 Abstract
Oxidative stress, or the production of oxygen-centered free radicals, has been hypothesized as the major source of DNA damage that can lead to a variety of diseases including cancer. It is known that 8-hydroxy-deoxyguanosine (8-oxo-dG) is a useful biomarker of oxidative DNA damage. Our recent data showed that JWA, initially being cloned as a novel cell differentiation-associated gene, was also actively responsive to environmental stressors, such as heat-shock, oxidative stress and so on. In the present study, we have applied a modified comet assay and bacterial repair endonucleases system (endonuclease III and formamidopyrimidine glycosylase) to investigate if JWA is involved in hydrogen peroxide (H
2O 2)-induced DNA damage and repair in K562 and MCF-7 cells, and to demonstrate if the damage is associated with 8-oxo-dG. The results from the comet assay have shown that the average tail length and the percentage of the cells with DNA tails are greatly induced by H 2O 2 treatment and further significantly enhanced by the post-treatment of repair endonucleases. The H 2O 2-induced 8-oxo-dG formation in K562 and MCF-7 cells is dose-dependent. In addition, the data have clearly demonstrated that JWA gene expression is actively induced by H 2O 2 treatment in K562 and MCF-7 cells. The results suggest that JWA can be regulated by oxidative stress and is actively involved in the signal pathways of oxidative stress in the cells. Keywords bacterial repair endonuclease cell culture comet assay oxidative damage References Martindale, J.L., Holbrook, N.J. 2002 Cellular response to oxidative stress Signaling for suicide and survival J. Cell. Physiol. 192 1 15 Google Scholar Finkel, T, Holbrook, N.J. 2000 Oxidants, oxidative stress and the biology of ageing Nature 408 239 247 Google Scholar Kasai, H., Crain, P.F., Kuchino, Y., Nishimura, S., Ootsuyama, A, Tanooka, H. 1986 Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair Carcinogenesis 7 1849 1851 Google Scholar Sen, P, Costa, M. 1986 Incidence and localization of sister chromatid exchanges induced by nickel and chromium compounds Carcinogenesis 7 1527 1533 Google Scholar Sugiyama, M., Wang, X.M., Costa, M. 1986 Comparison cytotoxicity of DNA lesions mouse induced by calcium chromate in human and hamster cell lines Cancer Res 46 4547 4551 Google Scholar Termini, J. 2000 Hydroperoxide-induced DNA damage mutations Mutat Res. 450 107 124 Google Scholar Schraufstatter, I.U., Hyslop, P.A., Hinshaw, D.B., Spragg, R.G., Sklar, L.A., Cochrane, C.G. 1986 Hydrogen peroxideinduced injury of cells and its prevention by inhibitors of poly(ADP-ribose) polymerase. Proc. Natl. Acad. Sci. USA 83 4908 4912 Google Scholar Dizdaroglu, M., Nackerdien, Z., Chao, B.C., Gajewski, E., Rao, G. 1991 Chemical nature of in vivo DNA base damage in hydrogen peroxide-treated mammalian cells Arch. Biochem Biophys 285 388 390 Google Scholar Dizdaroglu, M. 1992 Oxidative damage to DNA in mammalian chromatin Mutat Res. 275 331 342 Google Scholar
Zhou J.W., Di Y.P., Zhao Y.H. and Wu R., A novel cytoskeleton associate gene–cloning, identification, sequencing, regulation of expression and tissue distribution of JWA. In: Ye X.S., Shen B.F. and Tang X.F. (Eds), Investigation on Cell Modulation: Signal Transduction Apoptosis and Gene Expression. Military Medical Sciences Press, Beijing, China, 1999, pp. 110–119.
Cao, H.X., Xia, W., Shen, Q., Lu, H., Ye, J., Li, A.P., Zou, C.P., Zhou, J.W. 2002 The apoptosis role of JWA in acute promyelocytic leukemia cell differentiation 12-tetradecanoylphorbol-13-acetate triggered by retinoic acid and arsenic trioxide Chinese Sci Bull. 47 834 838 Google Scholar Xia, W., Zhou, J.W., Cao, H.X., Zou, C.P., Wang, C.Y., Shen, Q., Lu, H. 2001 The function relationship between structure of JWA in the modulation of cell differentiation Chinese Sci Bull 46 2063 2206 Google Scholar Bailly, V., Verly, W.G. 1987 Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst. J. Biochem 242 565 572 Google Scholar Collins, A.R., Duthie, S.J, Dobson, V.L. 1993 Direct enzymatic detection of endogenous base damage in human lymphocyte DNA Carcinogenesis 14 1733 1735 Google Scholar Katcher, H.L, Wallace, S.S. 1983 Characterization of the Escherichia coli X-ray endonuclease, endonuclease III Biochemistry 22 4071 4081 PubMed Google Scholar Krokan, H.E., Standal, R., Slupphaug, G. 1997 DNA glycosylases in the base excision repair J. Biochem 325 1 16 Google Scholar Cheng, K.C., Cahill, D.S., Kasai, H., Nishimura, S., Loeb, L.A. 1992 8-hydroxyguanine, an abundant form of oxidative DNA damage, causes Gâ€“T and Aâ€“C substitutions J. Biol. Chem 267 166 172 Google Scholar Moriya, M., Ou, C., Bodepudi, V., Johnson, F., Takeshita, M., Grollman, A.P. 1991 Site-specific mutagenesis using A gapped duplex vector: A study of translesion synthesis past 8-oxodeoxyguanosine in E coli. Mutat. Res. 254 281 288 Google Scholar Shibutani, S., Takeshita, M., Grollman, A.P. 1991 Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG Nature 349 431 434 Google Scholar Evans, M.D., Podmore, I.D., Daly, G.J., Perrett, D., Lunec, J., Herbert, K.E. 1995 Detection of purine lesions in cellular DNA using single cell gel electrophoresis with Fpg protein Biochem. Soc. Trans. 23 434 441 Google Scholar Singh, N., McCoy, M., Tice, R., Schneider, L. 1988 A simple technique for quantitation of low levels of DNA damage in individual cells Exp. Cell Res. 175 184 191 Google Scholar Michaels, M.L., Cruz, C., Grollman, A.P., Miller, J.H. 1992 Evidence that MutY and MutM combine to prevent mutations by an oxidatively damaged form of guanine in DNA Proc. Natl. Acad. Sci. USA 89 7022 7025 Google Scholar Kasai, H., Nishimura, S. 1984 Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents Nucleic Acids Res. 12 2137 2145 Google Scholar Shigenaga, M.K., Gimeno, C.J., Ames, B.N. 1989 Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of in vivo oxidative DNA damage Proc. Natl. Acad. Sci. USA 86 9697 9701 Google Scholar Ostling, O., Johanson, K.J. 1984 Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells Biochem. Biophys. Res. Commun. 123 291 298 Google Scholar Tchou, J., Kasai, H., Shibutani, S., Chung, M.-H., Laval, J., Grollman, A.P., Nishimura, S. 1991 8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity Proc. Natl. Acad. Sci. USA 88 4690 4694 Google Scholar Chen, H.R., Li, A.Q., Li, A.P., Zhou, J.W. 2004 JWA protein binds to Î±-tubulin in PC12 cells Chinese Sci. Bull. 49 467 471 Google Scholar Li, A.Q., Li, A.P., Mao, W.G., Chen, H.R., Huang, S., Qi, H., Ye, J., Zhang, Z.D., Wang, X.R., Sun, F., Zou, C.P., Zhou, J.W. 2003 JWA, a novel microtubule associated protein, regulates homeostasis of intracellular amino acids in PC12 cells Chinese Sci. Bull. 48 1535 1541 Google Scholar Boiteux, S., Gajewski, E., Laval, J., Dizdaroglu, M. 1992 Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): Excision of purine lesions in DNA produced by ionizing radiation or photosensitization Biochemistry 31 106 110 Google Scholar Dherin, C., Gasparutto, D., O’Connor, T.R., Cadet, J., Boiteu, S. 2004 Excision by the human methylpurine DNA N-glycosylase of cyanuric acid, a stable and mutagenic oxidation product of 8-oxo-7,8-dihydroguanine Int. J. Radiat. Biol. 80 21 27 Google Scholar Copyright information
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