AGE

, Volume 35, Issue 1, pp 103–115

Aging and vascular dysfunction: beneficial melatonin effects

  • Luigi Fabrizio Rodella
  • Gaia Favero
  • Claudia Rossini
  • Eleonora Foglio
  • Francesca Bonomini
  • Russel J. Reiter
  • Rita Rezzani
Article

DOI: 10.1007/s11357-011-9336-z

Cite this article as:
Rodella, L.F., Favero, G., Rossini, C. et al. AGE (2013) 35: 103. doi:10.1007/s11357-011-9336-z

Abstract

Aging is characterized by a progressive deterioration of physiological functions and metabolic processes. In aging and in diseases associated with the elderly, the loss of cells in vital structures or organs may be related to several factors. Sirtuin1 (SIRT1) is a member of the sirtuin family of protein deacetylases involved in life span extension; however, its involvement in the aging is not yet completely defined. Recently, melatonin, a pleiotropic molecule, shown to activate SIRT1 in primary neurons of young animals, as well as in aged neurons of a murine model of senescence. Melatonin is known to modulate oxidative stress-induced senescence and pro-survival pathways. We treated 6- and 15-week-old apolipoprotein E (APOE)-deficient mice (APOE 6w and 15w) with two melatonin formulations (FAST and RETARD) to evaluate their anti-aging effect. Morphological changes in vessels (aortic arch) of APOE mice were evaluated SIRT1, p53, endothelial nitric oxide synthase (eNOS), and endothelin-1 (ET-1) markers. We demonstrate that SIRT1 and eNOS decresed in APOE mice between 6 and 15 weeks and that aging induced an elevated expression of p53 and ET-1 in APOE animals. Melatonin improved the impairment of endothelial damage and reduced loss of SIRT1 and eNOS decreasing p53 and ET-1 expression. The RETARD melatonin preparation caused a greater improvement of vessel cytoarchitecture. In summary, we indicate that SIRT1-p53-eNOS axis as one of the important marker of advanced vascular dysfunctions linked to aging. Finally, we suggest that extended-release melatonin (RETARD) provides a more appropriate option for contrasting these dysfunctions compared with rapid release melatonin (FAST) administration.

Keywords

Melatonin Atherosclerosis Aging Endothelial cells 

Copyright information

© American Aging Association 2011

Authors and Affiliations

  • Luigi Fabrizio Rodella
    • 1
  • Gaia Favero
    • 1
  • Claudia Rossini
    • 1
  • Eleonora Foglio
    • 1
  • Francesca Bonomini
    • 1
  • Russel J. Reiter
    • 2
  • Rita Rezzani
    • 1
  1. 1.Department of Biomedical Sciences and Biotechnologies, Section of Human AnatomyUniversity of BresciaBresciaItaly
  2. 2.Department of Cellular and Structural BiologyUniversity of Texas Health Science CenterSan AntonioUSA

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