Article

AGE

, Volume 33, Issue 1, pp 15-31

Open Access This content is freely available online to anyone, anywhere at any time.

Caloric restriction or resveratrol supplementation and ageing in a non-human primate: first-year outcome of the RESTRIKAL study in Microcebus murinus

  • Alexandre Dal-PanAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
  • , Jérémy TerrienAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
  • , Fabien PifferiAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
  • , Roger BotallaAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
  • , Isabelle HardyAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
  • , Julia MarchalAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
  • , Alexandre ZaharievAffiliated withInstitut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS Université Louis Pasteur
  • , Isabelle CheryAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire NaturelleInstitut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS Université Louis Pasteur
  • , Philippe ZizzariAffiliated withCentre de Psychiatrie et Neuroscience, UMR 894 Inserm, Faculté de Médecine, Université Paris Descartes
    • , Martine PerretAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle
    • , Jean Luc PicqAffiliated withLaboratoire de Psychopathologie et Neuropsychologie, UFR Psychologie, Université Paris 08
    • , Jacques EpelbaumAffiliated withCentre de Psychiatrie et Neuroscience, UMR 894 Inserm, Faculté de Médecine, Université Paris Descartes
    • , Stéphane BlancAffiliated withInstitut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS Université Louis Pasteur
    • , Fabienne AujardAffiliated withMécanismes Adaptatifs et Evolution, UMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle Email author 

Abstract

A life-long follow-up of physiological and behavioural functions was initiated in 38-month-old mouse lemurs (Microcebus murinus) to test whether caloric restriction (CR) or a potential mimetic compound, resveratrol (RSV), can delay the ageing process and the onset of age-related diseases. Based on their potential survival of 12 years, mouse lemurs were assigned to three different groups: a control (CTL) group fed ad libitum, a CR group fed 70% of the CTL caloric intake and a RSV group (200 mg/kg.day–1) fed ad libitum. Since this prosimian primate exhibits a marked annual rhythm in body mass gain during winter, animals were tested throughout the year to assess body composition, daily energy expenditure (DEE), resting metabolic rate (RMR), physical activity and hormonal levels. After 1 year, all mouse lemurs seemed in good health. CR animals showed a significantly decreased body mass compared with the other groups during long day period only. CR or RSV treatments did not affect body composition. CR induced a decrease in DEE without changes in RMR, whereas RSV induced a concomitant increase in DEE and RMR without any obvious modification of locomotor activity in both groups. Hormonal levels remained similar in each group. In summary, after 1 year of treatment CR and RSV induced differential metabolic responses but animals successfully acclimated to their imposed diets. The RESTRIKAL study can now be safely undertaken on a long-term basis to determine whether age-associated alterations in mouse lemurs are delayed with CR and if RSV can mimic these effects.

Keywords

Ageing Food restriction Resveratrol Energy balance Biomarkers Doubly labelled water method