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Comparative study on the impact of copper sulphate and copper nitrate on the detoxification mechanisms in Typha latifolia

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Abstract

The present study focused on cupric sulphate and cupric nitrate uptake in Typha latifolia and the impact of these copper species on the plant’s detoxification capacity. When the plants were exposed to 10, 50 and 100 μM cupric sulphate or cupric nitrate, copper accumulation in T. latifolia roots and shoots increased with rising concentration of the salts. Shoot to root ratios differed significantly depending on the form of copper supplementation, e.g. if it was added as cupric (II) sulphate or cupric (II) nitrate. After incubation with 100 μM of cupric sulphate, up to 450 mg Cu/kg fresh weight (FW) was accumulated, whereas the same concentration of cupric nitrate resulted in accumulation of 580 mg/kg FW. Furthermore, significant differences in the activity of some antioxidative enzymes in Typha roots compared to the shoots, which are essential in the plant’s reaction to cope with metal stress, were observed. The activity of peroxidase (POX) in roots was increased at intermediate concentrations (10 and 50 μM) of CuSO4, whereas it was inhibited at the same Cu(NO3)2 concentrations. Ascorbate peroxidase (APOX) and dehydroascorbate reductase (DHAR) increased their enzyme activity intensely, which may be an indication for copper toxicity in T. latifolia plants. Besides, fluorodifen conjugation by glutathione S-transferases (GSTs) was increased up to sixfold, especially in roots.

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References

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Briat JF, Lebrun M (1999) Plant responses to metal toxicity. Comp R Acad Sci Paris 322:43–54

    Article  CAS  Google Scholar 

  • Cuypers A, Vangronseld J, Clijters H (1999) The chemical behavior of heavy metals plays a prominent role in the induction of oxidative stress. Free Rad Res 31:39–43

    Article  Google Scholar 

  • Dixon DP, Cole DJ, Edwards R (1998) Purification, regulation and cloning of a glutathione transferase (GST) from maize resembling the auxin—inducible type—III GSTs. Plant Molec Biol 36:75–87

    Article  CAS  Google Scholar 

  • Drazkiewicz M, Skórzynska-Polit E, Krupa Z (2003) Response of the ascorbate-glutathione cycle to excess copper in Arabidopsis thaliana (L). Plant Sci 164:195–202

    Article  CAS  Google Scholar 

  • Ducic T, Polle A (2005) Transport and detoxification of manganese and copper in plants. Braz J Plant Physiol 17:103–112

    Article  CAS  Google Scholar 

  • Gallagher DL, Johnston KM, Dietrich AM (2001) Fate and transport of copper-based crop protectants in plasticulture runoff and the impact of sedimentation as a best management practice. Water Res 35:2984–2994

    Article  CAS  Google Scholar 

  • García A, Baquedano FJ, Navarro P, Castillo FJ (1999) Oxidative stress induced by copper in sunflower plants. Free Rad Res 31:45–50

    Article  Google Scholar 

  • García JS, Gratão PL, Azevedo RA, Zezzi Arruda MA (2006) Metal contamination effects on sunflower (Helianthus annuus L.) growth and protein expression in leaves during development. J Agric Food Chem 54:8623–8630

    Article  Google Scholar 

  • Gunes A, Pilbeam D, Inal A, Coban S (2008) Influence of silicon on sunflower cultivars under drought stress. I: growth, antioxidant mechanisms and lipid peroxidation. Commun. Soil Sci. Plant Nutr 39:1885–1903

    CAS  Google Scholar 

  • Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    CAS  Google Scholar 

  • Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–11

    Article  CAS  Google Scholar 

  • Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219:1–14

    CAS  Google Scholar 

  • Hiramaya T, Alonso JM (2000) Ethylene captures a metal! Metal ions are involved in the ethylene perception and signal transduction. Plant Cell Physiol 41:548–555

    Article  Google Scholar 

  • Keen JH, William BJ (1978) Glutathione transferases. Catalysis of nucleophilic reactions of glutathione. J Biol Chem 253:5654–5657

    CAS  Google Scholar 

  • Küpper H, Kroneck PMH (2005) Heavy metal uptake by plants and cyanobacteria. Met Ions Biol Syst 44:97–142

    Google Scholar 

  • Küpper H, Götz B, Mijovilovich A, Küpper FC, Meyer-Klaucke W (2009) Complexation and toxicity of copper in higher plants. I. Characterization of copper accumulation, speciation, and toxicity in Crassula helmsii as a new copper accumulator. Plant Phys 151:702–714

    Article  Google Scholar 

  • Liao MT, Hedley MJ, Woolley DJ, Brooks RR, Nichols MA (2000) Copper uptake and translocation in chicory (Cichorium intybus L. cv. Grasslands Puna) and tomato (Lycopersicon esculentum Mill. cv. Rondy) plants grown in NFT system. I. Copper uptake and distribution in plants. Plant Soil 22:135–142

    Article  Google Scholar 

  • Lyubenova L, Nehnevajova E, Herzig R, Schröder P (2009) Response of antioxidant enzymes in Nicotiana tabacum clones during phytoextraction of heavy metals. Environ Sci Pollut Res 16:573–581

    Article  CAS  Google Scholar 

  • Marschner H (1995) Mineral nutrition in higher plants. Academic Press, San Diego, CA, pp 379–396

    Google Scholar 

  • Michalke B, Maier M, Tischer T, Anetzberger H, Gerdesmeyer I, Pellengahr C, Schulz CU, Schmitz C (2002) Mineralogische Analyse röntgenologisch definierter Verkalkungen bei Patienten mit chronischer Tendinosis calcarea der Rotatorenmanschette. Z Orthop Ihre Grenzgeb 140:399–403

    Article  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants, and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  Google Scholar 

  • Mocquot B, Vangronsveld J, Clijsters H, Mench M (1996) Copper toxicity in young maize (Zea mays L.) plants: effects on growth, mineral and chlorophyll contents, and enzyme activities. Plant Soil 182:287–300

    CAS  Google Scholar 

  • Nehnevajova E, Lyubenova L, Herzig R, Schröder P, Schwitzguébel J-P, Schmülling T (2012) Metal accumulation and response of antioxidant enzymes in seedlings and adult sunflower mutants with improved metal removal traits on a metal-contaminated soil. Environ Exp Bot 76:39–48

    Article  CAS  Google Scholar 

  • Nishizono H, Ishikawa H, Suziki S, Ishi F (1987) The role of the cell wall in the heavy metal tolerance of Athrium yokoskense. Plant Soil 101:15–20

    Article  CAS  Google Scholar 

  • Polle A, Krings B, Rennenberg H (1989) Superoxide dismutase activity in needles of Norwegian spruce trees (Picea abies L.). Plant Physiol 90:1310–1315

    Article  CAS  Google Scholar 

  • Quartacci MF, Pinzino C, Sgherri CLM, Dalla Vecchia F, Navari-Izzo F (2000) Growth in excess copper induces changes in the lipid composition and fluidity of PSII-enriched membranes in wheat. Physiol Plant 108:87–93

    Article  CAS  Google Scholar 

  • Rauser WE (2005) Phytochelatins and related peptides. Plant Physiol 109:1141–1149

    Article  Google Scholar 

  • Rocchetta I, Küpper H (2009) Chromium and copper induced inhibition of photosynthesis in Euglena gracilis analysed on the single-cell level by fluorescence kinetic microscopy. New Phytol 182:405–420

    Article  CAS  Google Scholar 

  • Scalla R, Roullet A (2002) Cloning and characterization of a glutathione S-transferase induced by a herbicide safener in barley (Hordeum vulgare). Physiol Plant 116:336–344

    Article  CAS  Google Scholar 

  • Shorrocks VM, Alloway BJ (1988) Copper in plant, animal and human nutrition. Copper Development Association, pp:1–109

  • Tietze F (1969) Enzymatic method for the quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522

    Article  CAS  Google Scholar 

  • van Assche F, Clijters H (1990) Effect of metal on enzyme activity in plants. Plant Cell Environ 3:195–206

    Article  Google Scholar 

  • Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655

    Article  CAS  Google Scholar 

  • Yruela I (2005) Copper in plants. Braz J Plant Physiol 17:145–156

    Article  CAS  Google Scholar 

  • Zhang J, Kirkham MB (1996) Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol 132:361–373

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was in part funded by an IAESTE/DAAD grant to HB and stimulated by the scientific activities within COST Action FA0995.

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Authors and Affiliations

  1. Department of Environmental Sciences, Research Unit Microbe-Plant Interactions, German Research Center for Environmental Health, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany

    Lyudmila Lyubenova & Peter Schröder

  2. Department of Theoretical and Applied Biology, Faculty of Biosciences, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

    Hanif Bipuah & Ebenezer Belford

  3. German Research Center for Environmental Health, Institute of Ecological Chemistry, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany

    Bernhard Michalke

  4. Department of Environmental Sciences, Research Unit Environmental Simulation, German Research Center for Environmental Health, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany

    Barbro Winkler

Authors
  1. Lyudmila Lyubenova
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  2. Hanif Bipuah
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  3. Ebenezer Belford
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  4. Bernhard Michalke
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  5. Barbro Winkler
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  6. Peter Schröder
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Correspondence to Peter Schröder.

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Responsible editor: Philippe Garrigues

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Lyubenova, L., Bipuah, H., Belford, E. et al. Comparative study on the impact of copper sulphate and copper nitrate on the detoxification mechanisms in Typha latifolia . Environ Sci Pollut Res 22, 657–666 (2015). https://doi.org/10.1007/s11356-014-3402-x

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  • DOI: https://doi.org/10.1007/s11356-014-3402-x

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