Environmental Science and Pollution Research

, Volume 21, Issue 4, pp 2977–2987

Low impact of phenanthrene dissipation on the bacterial community in grassland soil

Authors

  • Maïté Niepceron
    • Laboratoire de Microbiologie Signaux et MicroenvironnementUniversité de Rouen
    • Laboratoire M2C, UMR CNRS 6143Université de Rouen
  • Jérémie Beguet
    • INRA, UMR 1347 Agroécologie
  • Florence Portet-Koltalo
    • Laboratoire COBRA, UMR CNRS 6014Université de Rouen
  • Fabrice Martin-Laurent
    • INRA, UMR 1347 Agroécologie
    • INRA, Service de Séquençage et de Génotypage, SSG
  • Laurent Quillet
    • Laboratoire de Microbiologie Signaux et MicroenvironnementUniversité de Rouen
    • Laboratoire de Microbiologie Signaux et MicroenvironnementUniversité de Rouen
    • LMSM, Bâtiment IRESE B, UFR des SciencesUniversité de Rouen
Research Article

DOI: 10.1007/s11356-013-2258-9

Cite this article as:
Niepceron, M., Beguet, J., Portet-Koltalo, F. et al. Environ Sci Pollut Res (2014) 21: 2977. doi:10.1007/s11356-013-2258-9

Abstract

The effect of phenanthrene on the bacterial community was studied on permanent grassland soil historically presenting low contamination (i.e. less than 1 mg kg−1) by polycyclic aromatic hydrocarbons (PAHs). Microcosms of soil were spiked with phenanthrene at 300 mg kg−1. After 30 days of incubation, the phenanthrene concentration decreased rapidly until its total dissipation within 90 days. During this incubation period, significant changes of the total bacterial community diversity were observed, as assessed by automated-ribosomal intergenic spacer analysis fingerprinting. In order to get a deeper view of the effect of phenanthrene on the bacterial community, the abundances of ten phyla and classes (Actinobacteria, Acidobacteria, Bacteroidetes, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Verrucomicrobiales, Gemmatimonadetes, and Planctomycetes) were monitored by quantitative polymerase chain reaction performed on soil DNA extracts. Interestingly, abundances of some bacterial taxa significantly changed as compared with controls. Moreover, among these bacterial groups impacted by phenanthrene spiking, some of them presented the potential of phenanthrene degradation, as assessed by PAH-ring hydroxylating dioxygenase (PAH-RHDα) gene detection. However, neither the abundance nor the diversity of the PAH-RHDα genes was significantly impacted by phenanthrene spiking, highlighting the low impact of this organic contaminant on the functional bacterial diversities in grassland soil.

Keywords

Polycyclic aromatic hydrocarbonMicrocosmPhenanthreneA-RISAqPCRDioxygenase

Supplementary material

11356_2013_2258_MOESM1_ESM.doc (288 kb)
Fig. S1A-RISA fingerprinting from extracted DNA microcosms (n= 3) (DOC 524 kb) (DOC 288 kb)
11356_2013_2258_MOESM2_ESM.doc (254 kb)
Fig. S2Relative abundances of phylum-specific 16S rRNA genes in soil DNA during incubations with phenanthrene (DOC 254 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013