A protocol for assessing the effectiveness of oil spill dispersants in stimulating the biodegradation of oil
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- Prince, R.C. & Butler, J.D. Environ Sci Pollut Res (2014) 21: 9506. doi:10.1007/s11356-013-2053-7
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Dispersants are important tools in oil spill response. Taking advantage of the energy in even small waves, they disperse floating oil slicks into tiny droplets (<70 μm) that entrain in the water column and drift apart so that they do not re-agglomerate to re-form a floating slick. The dramatically increased surface area allows microbial access to much more of the oil, and diffusion and dilution lead to oil concentrations where natural background levels of biologically available oxygen, nitrogen, and phosphorus are sufficient for microbial growth and oil consumption. Dispersants are only used on substantial spills in relatively deep water (usually >10 m), conditions that are impossible to replicate in the laboratory. To date, laboratory experiments aimed at following the biodegradation of dispersed oil usually show only minimal stimulation of the rate of biodegradation, but principally because the oil in these experiments disperses fairly effectively without dispersant. What is needed is a test protocol that allows comparison between an untreated slick that remains on the water surface during the entire biodegradation study and dispersant-treated oil that remains in the water column as small dispersed oil droplets. We show here that when this is accomplished, the rate of biodegradation is dramatically stimulated by an effective dispersant, Corexit 9500®. Further development of this approach might result in a useful tool for comparing the full benefits of different dispersants.
KeywordsOil spill dispersantsCrude oilBiodegradationOil spill response
Dispersants are important options in the “tool box” for responding to marine oil spills. Developed after initial attempts to disperse oil with simple industrial cleaners (National Research Council 1989), current products are carefully blended mixtures of usually food-grade surfactants and solvents (National Research Council 2005) that can disperse a range of crude oils and refined products (Lessard and DeMarco 2000) over a wide range of conditions ranging from arctic (Belore et al. 2009) to tropical (Zahed et al. 2011).
Several laboratory protocols have been developed for testing the relative efficacy of different dispersants: the air current test (Mackay et al. 1978), the Warren Springs rolling flask test (Martinelli 1984), the swirling flask test (Clayton et al. 1993), the Exxon dispersant effectiveness test (Becker et al. 1993), and the baffled flask test (Venosa et al. 2002) among others. Comparisons of these tests with field and tank data have been reported by Clark et al. (2005), and the baffled flask test was used to compare eight potential dispersants during the response to the Deepwater Horizon spill (Venosa and Holder 2012).
Useful as they are, these are very small volume tests, and they are not amenable to studying long-term processes (days to weeks) such as biodegradation. In practice, dispersants are only used on significant slicks, and the tests use relatively high concentrations of oil to mimic this. In the field, the dispersed oil droplets (typically <70 μm) diffuse apart, dropping to concentrations below 1 ppm in hours to days (Delvigne 1993; Li et al. 2009; Lee et al. 2013), and they do not re-coalesce. Until now, laboratory tests have used volumes that keep the oil concentration in the many hundreds to many thousands of parts per million, and re-coalescence occurs in hours to days.
The ultimate purpose of oil spill dispersants is to dilute spilled oil so that indigenous microbes in the sea can consume the oil. Hydrocarbons are excellent sources of carbon and energy for those organisms, prokaryotic (Prince et al. 2010) and eukaryotic (Prince 2010), able to initiate biodegradation, but they lack biologically available nitrogen, phosphorus, and other essential elements for microbial growth. Fortunately, seawater usually provides enough of these nutrients if the hydrocarbons are in the few parts per million concentration or less, and biodegradation of dilute oil has a “half-life” of days to weeks (Hazen et al. 2010; Baelum et al. 2012; Prince et al. 2013).
Quantitatively demonstrating that oil dispersants do indeed stimulate oil biodegradation under controlled conditions has been a major challenge. Current protocols for testing the efficacy of dispersants generate dispersions that are far too concentrated for the available nitrogen, phosphorus, etc. in seawater to allow prompt biodegradation, and experiments are rarely carried out long enough for biodegradation to occur even if the nutrient problem is overcome by adding fertilizer (although see MacNaughton et al. 2003). Conversely, oil at the few parts per million level, where biodegradation is rapid, is dispersed fairly well by laboratory stirring even without adding dispersants, and dispersants have only a minimal stimulatory effect on biodegradation (Prince et al. 2013). Here, we describe experiments where the total oil available in the system is dilute enough that there are potentially enough nutrients in the seawater for rapid biodegradation, but where natural dispersion is slowed by enclosure of the oil in a floating boom unless dispersant is added. These concentrations approximate those found after the successful application of dispersants at sea (Lee et al. 2013), and are below levels where any measurable toxicity occurs, even in 48 (Americamysis bahia) or 96 h (Menidia beryllina) tests (Hemmer et al. 2011). Under these conditions, Corexit 9500, the dispersant used most widely following the Deepwater Horizon spill, dramatically stimulates the rate of oil biodegradation.
Upon sacrifice, the booms were carefully removed and the carboys extracted three times with methylene chloride. The glass booms were removed from their foam flotation collars and washed twice with methylene chloride. The methylene chloride extracts were collected with a pipette, evaporated carefully to a few milliliters, dried over anhydrous sodium sulfate, and then evaporated to approximately 1 ml, with care to prevent concentration to dryness. In the absence of dispersant, there were no detectable hydrocarbons in the bulk phase, while with dispersant, there was no oil remaining on the glass booms.
Oils were analyzed by gas chromatography coupled with mass spectrometry (Douglas et al. 1992). Hydrocarbon biodegradation was followed with respect to 17α(H),21β(H)-hopane as a conserved internal marker within the oil (Prince et al. 1994).
As far as we are aware, this is the first laboratory demonstration of a substantial and dramatic stimulation of the rate of biodegradation of crude oil by the addition of an oil dispersant. At first glance, this seems to contradict a substantial body of work that found only minimal stimulation at best (Van Hamme and Ward 1999; Lindstrom and Braddock 2002; Venosa and Holder 2007; Prince et al. 2013). We believe that this can be entirely attributed to the fact that in those experiments the relatively low concentrations of oil dispersed quite naturally, almost as well as with dispersants, albeit with slightly larger droplet size (Shaw and Reidy 1979). Since biodegradation is likely dependent on the surface area available for microbial colonization, there is only a small increase of accessibility in decreasing droplet size once small droplets have formed. In contrast, going from a floating slick to 70 μm droplets increases surface area by at least 20-fold, and the stimulation of biodegradation seen here is consistent with this increase.
Several things are noteworthy. The first is that the biodegradation of dispersed oil in these experiments is only marginally faster than it was in water collected at the same site under winter conditions, an apparent “half-life” of about 1 week at 21 °C (Fig. 2) compared with 11 days at 8 °C (Prince et al. 2013), which compares with 28 days (at 100 ppm oil) at 27.5 °C off the Penang, Malaysia shore (Zahed et al. 2011). The second is that these experiments used fresh seawater, not an acclimated inoculum (Venosa and Holder 2007; Campo et al. 2013), thus allowing the indigenous microbes to respond to the oil with the indigenous levels of biological nitrogen, phosphorus, and iron, etc. Next is the observation that the water under the floating slick in the undispersed experiments contained no detectable hydrocarbons (detection limit of the order of a few parts per billion for individual compounds), indicating that any hydrocarbons that dissolved out of the floating slick were promptly degraded by the indigenous microbes.
Our experiments offer the potential for developing a protocol for assessing the biodegradability of dispersed oil that might reveal differences between the biodegradability of dispersions generated by different dispersants. The tests described in the Introduction already offer a way of comparing the dispersion effectiveness of different commercial and experimental products. Building on our protocol, especially to assess and ensure its precision, might allow the development of tests for assessing whether particular formulations stimulate or transiently inhibit biodegradation (Varadaraj et al. 1995). As the protocol is developed, it will be important to ensure that the stirring is well controlled, since it is well known that mixing energy is a primary determinant of the differences seen in the current tests (Venosa et al. 2002; Clark et al. 2005). We use fresh seawater as inoculum because we do not have a clear idea of how to preserve an inoculum for repeated use. This may introduce variability, but attempts to maintain or preserve cultures also have their difficulties, most notably prolonged lag phases before biodegradation begins (Venosa and Holder 2007). More work is needed to determine the better option.
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