Environmental Science and Pollution Research

, Volume 19, Issue 7, pp 2498–2505

Effects of perfluorinated compounds on development of zebrafish embryos

15th International Symposium on Toxicity Assessment

DOI: 10.1007/s11356-012-0977-y

Cite this article as:
Zheng, XM., Liu, HL., Shi, W. et al. Environ Sci Pollut Res (2012) 19: 2498. doi:10.1007/s11356-012-0977-y

Abstract

Perfluorinated compounds (PFCs) have been widely used in industrial and consumer products and frequently detected in many environmental media. Potential reproductive effects of perfluorooctanesulfonate (PFOS), perfluorooctanoic acid (PFOA) and perfluorononanoic acid (PFNA) have been reported in mice, rats and water birds. PFOS and PFOA were also confirmed developing toxicants towards zebrafish embryos; however, the reported effect concentrations were contradictory. Polyfluorinated alkylated phosphate ester surfactants (including FC807) are precursor of PFOS and PFOA; however, there is no published information about the effects of FC807 and PFNA on zebrafish embryos. Therefore, this study was conducted to determine the effects of these four PFCs on zebrafish embryos. Normal fertilized zebrafish embryos were selected to be exposed to several concentrations of PFOA, PFNA, PFOS or FC807 in 24-well cell culture plates. A digital camera was used to image morphological anomalies of embryos with a stereomicroscope. Embryos were observed through matching up to 96-h post-fertilization (hpf) and rates of survival and abnormalities recorded. PFCs caused lethality in a concentration-dependent manner with potential toxicity in the order of PFOS > FC807 > PFNA > PFOA based on 72-h LC50. Forty-eight-hour post-fertilization pericardial edema and 72- or 96-hpf spine crooked malformation were all observed. PFOA, PFNA, PFOS and FC807 all caused structural abnormalities using early stages of development of zebrafish. The PFCs all retarded the development of zebrafish embryos. The toxicity of the PFCs was related to the length of the PFC chain and functional groups.

Keywords

PFNAPFOAPFOSFC807In ovoDevelopmental toxicity

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.State Key Laboratory of Pollution Control and Resource Reuse, School of the EnvironmentNanjing UniversityNanjingChina
  2. 2.Department of Veterinary Biomedical Sciences and Toxicology CentreUniversity of SaskatchewanSaskatoonCanada
  3. 3.Department of ZoologyMichigan State UniversityEast LansingUSA
  4. 4.Center for Integrative ToxicologyMichigan State UniversityEast LansingUSA
  5. 5.Zoology Department, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  6. 6.Department of Biology and ChemistryCity University of Hong KongHong KongChina
  7. 7.School of Biological SciencesUniversity of Hong KongHong KongChina
  8. 8.State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental ScienceXiamen UniversityXiamenChina