Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64
- D. P. SinghAffiliated withDepartment of Botany, Punjabi University Email author
- , J. I. S. KhattarAffiliated withDepartment of Botany, Punjabi University
- , J. NaddaAffiliated withDepartment of Botany, Punjabi University
- , Y. SinghAffiliated withDepartment of Botany, Punjabi University
- , A. GargAffiliated withDepartment of Chemistry, Punjab Agricultural University
- , N. KaurAffiliated withPlant Pathology and Microbiology Lab, Institute of Himalayan Bioresource Technology (CSIR)
- , A. GulatiAffiliated withPlant Pathology and Microbiology Lab, Institute of Himalayan Bioresource Technology (CSIR)
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Background, aim, and scope
Indiscriminate use of insecticides leads to environmental problems and poses a great threat to beneficial microorganisms. The aim of the present work was to study chlorpyrifos degradation by a rice field cyanobacterium Synechocystis sp. strain PUPCCC 64 so that the organism is able to reduce insecticide pollution in situ.
Material and methods
The unicellular cyanobacterium isolated and purified from a rice field was identified by partial 16S rRNA gene sequence as Synechocystis sp. strain PUPCCC 64. Tolerance limit of the organism was determined by studying its growth in graded concentrations (2.5–20 mg/L) of chlorpyrifos. Chlorpyrifos removal was studied by its depletion from the insecticide supplemented growth medium, and its biodegradation products were identified in the cell extract, biomass wash, and growth medium.
Results and discussion
The organism tolerated chlorpyrifos up to 15 mg/L. Major fraction of chlorpyrifos was removed by the organism during the first day followed by slow uptake. Biomass, pH, and temperature influenced the insecticide removal and the organism exhibited maximum chlorpyrifos removal at 100 mg protein/L biomass, pH 7.0, and 30°C. The cyanobacterium metabolized chlorpyrifos producing a number of degradation products as evidenced by GC-MS chromatogram. One of the degradation products was identified as 3,5,6-trichloro-2-pyridinol.
Conclusion and recommendations
Present study reports the biodegradation of chlorpyrifos by Synechocystis sp. Biodegradation of the insecticide by the cyanobacterium is significant as it can be biologically removed from the environment. The cyanobacterium may be used for bioremediation of chlorpyrifos-contaminated soils.
KeywordsBiodegradation Bioremediation Chlorpyrifos Cyanobacterium Synechocystis 3,5,6-trichloro-2-pyridinol
- Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64
Environmental Science and Pollution Research
Volume 18, Issue 8 , pp 1351-1359
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- Author Affiliations
- 1. Department of Botany, Punjabi University, Patiala, 147 002, Punjab, India
- 2. Department of Chemistry, Punjab Agricultural University, Ludhiana, 141 004, Punjab, India
- 3. Plant Pathology and Microbiology Lab, Institute of Himalayan Bioresource Technology (CSIR), Post Box No. 6, Palampur, 176 061, Himachal Pradesh, India