Research Article

Environmental Science and Pollution Research

, Volume 17, Issue 7, pp 1355-1361

First online:

Removal of 4-chlorobenzoic acid from spiked hydroponic solution by willow trees (Salix viminalis)

  • Kamila DeaversAffiliated withDepartment of Environmental Engineering, Technical University of DenmarkInstitute of Organic Chemistry and Biochemistry
  • , Tomas MacekAffiliated withInstitute of Organic Chemistry and Biochemistry
  • , Ulrich G. KarlsonAffiliated withDepartment of Environmental Chemistry and Microbiology, National Environmental Research Institute, University of Aarhus
  • , Stefan TrappAffiliated withDepartment of Environmental Engineering, Technical University of Denmark Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Background

Chlorobenzoic acids (CBA) are intermediate products of the aerobic microbial degradation of PCB and several pesticides. This study explores the feasibility of using basket willows, Salix viminalis, to remove 4-CBA from polluted sites, which also might stimulate PCB degradation.

Methods

The removal of 4-CBA by willow trees was investigated with intact, septic willow trees growing in hydroponic solution and with sterile cell suspensions at concentrations of 5 mg/L and 50 mg/L 4-CBA. Nutrient solutions with different levels of ammonium and nitrate were prepared to achieve different pH levels. The concentration of 4-CBA was tracked over time and quantified by HPLC.

Results and discussion

At the low level of 4-CBA (5 mg/L), willows removed 70% (pH 4.2) to 90% (pH 6.8), while 48% (pH 4.2) to 52% (pH 6.8) of the water was transpired. At the high 4-CBA level (50 mg/L), the pH varied between 4.4 and 4.6, and 10% to 30% of 4-CBA was removed, but only 5% to 9% of the water. In sterile cell suspensions, removal of 4-CBA by fresh biomass was much higher than removal by dead biomass.

Conclusions

The results indicate that 4-CBA is toxic to willow trees at 50 mg/L. The removal of 4-CBA from solution is by both passive processes (uptake with water, sorption to plant tissue) and metabolic processes of the plants.

Recommendations and outlook

Plants, such as willow trees, might assist in the degradation of PCB and their degradation products CBA.

Keywords

Chlorobenzoic acid Degradation Metabolism PCB Plants Willows