Molecular Imaging and Biology

, Volume 15, Issue 1, pp 19–27

Micro-Autoradiographic Assessment of Cell Types Contributing to 2-Deoxy-2-[18F]Fluoro-d-Glucose Uptake During Ventilator-Induced and Endotoxemic Lung Injury

  • Dalia Saha
  • Kazue Takahashi
  • Nicolas de Prost
  • Tilo Winkler
  • Miguel Pinilla-Vera
  • Rebecca M. Baron
  • Marcos F. Vidal Melo
Research Article

DOI: 10.1007/s11307-012-0575-x

Cite this article as:
Saha, D., Takahashi, K., de Prost, N. et al. Mol Imaging Biol (2013) 15: 19. doi:10.1007/s11307-012-0575-x

Abstract

Purpose

The aim of the study was to use micro-autoradiography to investigate the lung cell types responsible for 2-deoxy-2-[18F]fluoro-d-glucose (FDG) uptake in murine models of acute lung injury (ALI).

Procedures

C57/BL6 mice were studied in three groups: controls, ventilator-induced lung injury (VILI), and endotoxin. VILI was produced by high tidal volumes and zero end-expiratory pressure and endotoxin ALI, by intranasal administration. Following FDG injection, the lungs were processed and exposed to autoradiographic emulsion. Grain density over cells was used to quantify FDG uptake.

Results

Neutrophils, macrophages, and type 2 epithelial cells presented higher grain densities during VILI and endotoxin ALI than controls. Remarkably, cell grain density in specific cell types was dependent on the injury mechanism. Whereas macrophages showed high grain densities during endotoxin ALI, similar to those exhibited by neutrophils, type 2 epithelial cells demonstrated the second highest grain density (with neutrophils as the highest) during VILI.

Conclusions

In murine models of VILI and endotoxin ALI, FDG uptake occurs not only in neutrophils but also in macrophages and type 2 epithelial cells. FDG uptake by individual cell types depends on the mechanism underlying ALI.

Key Words

Micro-autoradiography 2-Deoxy-2-[18F]fluoro-d-glucose Positron emission tomography Ventilator-induced lung injury Acute lung injury Endotoxin lung injury Neutrophil Macrophage Type 2 epithelial cell Lung 

Copyright information

© World Molecular Imaging Society 2012

Authors and Affiliations

  • Dalia Saha
    • 1
  • Kazue Takahashi
    • 2
  • Nicolas de Prost
    • 1
  • Tilo Winkler
    • 1
  • Miguel Pinilla-Vera
    • 3
  • Rebecca M. Baron
    • 3
  • Marcos F. Vidal Melo
    • 1
  1. 1.Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General HospitalHarvard Medical SchoolBostonUSA
  2. 2.Program of Developmental Immunology, Department of Pediatrics, Massachusetts General HospitalHarvard Medical SchoolBostonUSA
  3. 3.Department of Medicine (Pulmonary and Critical Care), Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA

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