Molecular Imaging and Biology

, Volume 14, Issue 3, pp 376–383

Correlation of the Ga-68-Bombesin Analog Ga-68-BZH3 with Receptors Expression in Gliomas as Measured by Quantitative Dynamic Positron Emission Tomography (dPET) and Gene Arrays

  • Ludwig G. Strauss
  • Dirk Koczan
  • Marcel Seiz
  • Jochen Tuettenberg
  • Kirsten Schmieder
  • Leyun Pan
  • Caixia Cheng
  • Antonia Dimitrakopoulou-Strauss
Research Article

DOI: 10.1007/s11307-011-0508-0

Cite this article as:
Strauss, L.G., Koczan, D., Seiz, M. et al. Mol Imaging Biol (2012) 14: 376. doi:10.1007/s11307-011-0508-0

Abstract

Purpose

The kinetics of Ga-68-BZH3, a Ga-68-bombesin analog, was compared to molecular biological data obtained from gene arrays in seven patients with a recurrent glioma. The primary aim of this study was the correlation of receptor expression and tracer kinetics.

Procedures

Dynamic positron emission tomography studies were performed and the data were analyzed by a volume-of-interest technique using a two-tissue compartment model as well as a non-compartment model. Gene array data were obtained from gene array analysis of tumor tissue samples.

Results

The correlation analysis revealed a significant nonlinear correlation of r = 0.89 (p < 0.03) for k1 and BB2 (gastrin-releasing peptide receptor). BB1 and BB3 were not significantly correlated with k1. vb and k3 were not significantly correlated with the expression data of the receptors on the p < 0.05 level.

Conclusions

The parameter k1 is correlated with the expression of BB2 based on gene array data. The quantitative analysis of the Ga-68-BZH3 kinetics can be used to predict the receptor expression of BB2 in gliomas based on k1 of the compartment analysis. However, this study is limited to the expression data on the mRNA level and further studies are needed to assess the correlation of gene expression on the protein level.

Key words

PETGa-68-bombesinGRPRNeuromedin BBombesin subtype 3BB1BB2BB3Gene arrayGlioma

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2011

Authors and Affiliations

  • Ludwig G. Strauss
    • 1
  • Dirk Koczan
    • 2
  • Marcel Seiz
    • 3
  • Jochen Tuettenberg
    • 4
  • Kirsten Schmieder
    • 5
  • Leyun Pan
    • 1
  • Caixia Cheng
    • 1
  • Antonia Dimitrakopoulou-Strauss
    • 1
  1. 1.Medical PET Group—Biological Imaging [E060-1], CCU Nuclear MedicineGerman Cancer Research CenterHeidelbergGermany
  2. 2.Institute of ImmunologyUniversity of RostockRostockGermany
  3. 3.Clinical Department of NeurosurgeryMedical University InnsbruckInnsbruckAustria
  4. 4.Department of NeurosurgeryKlinikum Idar-Oberstein GmbHIdar-ObersteinGermany
  5. 5.Department of NeurosurgeryUniversity of Heidelberg, University Medical Center MannheimMannheimGermany