In Vivo Imaging of Lymph Node Migration of MNP- and 111 In-Labeled Dendritic Cells in a Transgenic Mouse Model of Breast Cancer (MMTV-Ras )
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The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model.
Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or 111In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data.
DC labeling, both with MNPs and with 111In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and 111In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs.
MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
- In Vivo Imaging of Lymph Node Migration of MNP- and 111In-Labeled Dendritic Cells in a Transgenic Mouse Model of Breast Cancer (MMTV-Ras)
Molecular Imaging and Biology
Volume 14, Issue 2 , pp 183-196
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- Dendritic cells
- In vivo imaging
- Vaccine protocol
- Breast cancer
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- Author Affiliations
- 1. Department of Biomedical Sciences and Technologies, Section of Radiological Sciences, University of Milan, via di Rudinì 8, 20142, Milan, Italy
- 2. Centre of Molecular and Cellular Imaging – IMAGO, University of Milan, via Fratelli Cervi 93, 20090, Segrate, Milan, Italy
- 3. Department of Clinical Sciences, Chair of Immunology, University of Milan, via GB Grassi 74, Milan, Italy
- 4. Pharmacology Department, BU Oncology, Nerviano Medical Sciences, viale Pasteur 10, 20014, Nerviano, Milan, Italy
- 5. Pathology Unit, Department of Medicine, Surgery and Dentistry, University of Milan Medical School, IRCCS Ca’ Granda – Ospedale Maggiore Policlinico Foundation, via Sforza, 28, 20122, Milan, Italy
- 6. Electronic and Information Technology Department, Politecnico di Milano, via Golgi 40, 20133, Milan and INFN, Milan Division, Milan, 20133, Italy
- 7. Department of Biomedical Sciences and Technologies, University of Milan, via Fratelli Cervi 93, 20090, Segrate, Milan, Italy
- 8. IRCCS Don C. Gnocchi Foundation, Via Capecelatro 66, 20148, Milan, Italy