Molecular Imaging and Biology

, Volume 13, Issue 6, pp 1157–1162

Longitudinal Molecular Imaging with Single Cell Resolution of Disseminated Ovarian Cancer in Mice with a LED-based Confocal Microendoscope

  • Sakib F. Elahi
  • Zhongyao Liu
  • Kathryn E. Luker
  • Richard S. Kwon
  • Gary D. Luker
  • Thomas D. Wang
Research Article

DOI: 10.1007/s11307-010-0455-1

Cite this article as:
Elahi, S.F., Liu, Z., Luker, K.E. et al. Mol Imaging Biol (2011) 13: 1157. doi:10.1007/s11307-010-0455-1

Abstract

Purpose

We engineered a flexible fiber-optic microendoscope for longitudinal optical imaging studies in a mouse model of disseminated ovarian cancer.

Procedures

The microendoscope delivers 470 nm excitation light from a light-emitting diode through a fiber-optic bundle with outer diameter of 680 μm. Optics were optimized to maximize power and lateral resolution. We used this instrument to repetitively monitor intraperitoneal growth of HeyA8 ovarian cancer cells stably transduced with green fluorescent protein over 4 weeks.

Results

The microendoscope achieves 0.7 mW power and lateral resolution of 4 μm. Initial in vivo imaging studies visualized single cells and small clusters of malignant cells with subsequent studies showing tumor masses and vasculature. We also resolved single cells within intraperitoneal tumor masses.

Conclusions

These studies establish microendoscope technology with single cell resolution for minimally-invasive, longitudinal imaging in living animals. This technology will advance future molecular imaging studies of ovarian cancer and other diseases.

Key words

Confocal Microscopy Fluorescence In vivo LED Fiber bundle Small animal imaging Ovarian cancer Xenograft Zemax® 

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010

Authors and Affiliations

  • Sakib F. Elahi
    • 1
  • Zhongyao Liu
    • 2
  • Kathryn E. Luker
    • 3
  • Richard S. Kwon
    • 4
  • Gary D. Luker
    • 5
  • Thomas D. Wang
    • 6
  1. 1.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA
  2. 2.Department of Medicine, Division of GastroenterologyUniversity of MichiganAnn ArborUSA
  3. 3.Center for Molecular Imaging, Department of RadiologyUniversity of MichiganAnn ArborUSA
  4. 4.Department of Medicine, Division of GastroenterologyUniversity of MichiganAnn ArborUSA
  5. 5.Center for Molecular Imaging, Departments of Radiology and Microbiology and ImmunologyUniversity of MichiganAnn ArborUSA
  6. 6.Department of Medicine, Division of Gastroenterology, Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA