In Situ Labeling and Magnetic Resonance Imaging of Transplanted Human Hepatic Stem Cells
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The purpose is to address the problem in magnetic resonance imaging (MRI) of contrast agent dilution.
In situ magnetic labeling of cells and MRI were used to assess distribution and growth of human hepatic stem cells (hHpSCs) transplanted into severe combined immunodeficiency (SCID)/non-obese diabetic (NOD) mice. It was done with commercially available magnetic microbeads coupled to an antibody to a surface antigen, epithelial cell adhesion molecule (EpCAM), uniquely expressed in the liver by hepatic progenitors.
We validated the microbead connection to cells and related MRI data to optical microscopy observations in order to develop a means to quantitatively estimate cell numbers in the aggregates detected. Cell counts of hHpSCs at different times post-transplantation revealed quantifiable evidence of cell engraftment and expansion.
This magnetic labeling methodology can be used with any antibody coupled to a magnetic particle to target any surface antigen that distinguishes transplanted cells from host cells, thus facilitating studies that define methods and strategies for clinical cell therapy programs.
- In Situ Labeling and Magnetic Resonance Imaging of Transplanted Human Hepatic Stem Cells
Molecular Imaging and Biology
Volume 13, Issue 5 , pp 911-922
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Magnetic resonance imaging (MRI)
- Cell labeling and tracking
- Human hepatic stem cells
- Cell therapies
- Industry Sectors
- Author Affiliations
- 1. Department of Cell and Molecular Physiology, UNC School of Medicine, Chapel Hill, NC, 27599, USA
- 7. SciKon, Suites 3–4, Cape Fear Bldg., 3200 Chapel Hill-Nelson Blvd, Research Triangle Park, NC, 27709, USA
- 8. Laboratoriet för Klinisk kemi och Transfusionsmedicin, Skene Lasarett Varbergsvägen, 50511 81, Skene, Sweden
- 2. Department of Biomedical Engineering, UNC School of Medicine, Chapel Hill, NC, 27599, USA
- 3. Program in Molecular Biology and Biotechnology, UNC School of Medicine, Chapel Hill, NC, 27599, USA
- 5. Room 34, UNC School of Medicine, 101 Mason Farm Rd, Chapel Hill, NC, 27599, USA
- 4. Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Durham, NC, 27708, USA
- 6. Department of Bioengineering, University of Utah, 72 S. Central Campus Drive, RM 2750, Salt Lake City, UT, 84112-9458, USA