Molecular Imaging and Biology

, Volume 13, Issue 4, pp 712–720

Localization of Deoxyglucose and Annexin A5 in Experimental Atheroma Correlates with Macrophage Infiltration but not Lipid Deposition in the Lesion

Authors

  • Yan Zhao
    • Department of Nuclear Medicine, Graduate School of MedicineHokkaido University
  • Songji Zhao
    • Department of Tracer Kinetics and Bioanalysis, Graduate School of MedicineHokkaido University
    • Central Institute of Isotope ScienceHokkaido University
  • William H. Strauss
    • Department of Nuclear MedicineMemorial Sloan-Kettering Cancer Center
  • Francis G. Blankenberg
    • Department of Pediatric RadiologyStanford University School of Medicine
  • Nagara Tamaki
    • Department of Nuclear Medicine, Graduate School of MedicineHokkaido University
Research Article

DOI: 10.1007/s11307-010-0389-7

Cite this article as:
Zhao, Y., Zhao, S., Kuge, Y. et al. Mol Imaging Biol (2011) 13: 712. doi:10.1007/s11307-010-0389-7

Abstract

Purpose

The aim of this study was to understand the relationship of lipid deposition to the macrophage content, macrophage metabolism, and apoptosis in plaque. We compared the uptake of 2-deoxy-2-fluoro-D-[14C]glucose ([14C]FDG) and [99mTc]HYNIC-annexin V ([99mTc]annexin A5) with the lesion histology in apolipoprotein E knockout (apoE−/−) mice.

Procedures

Male apoE−/− mice (n = 9) were injected with [14C]FDG and [99mTc]annexin A5. Cryostat sections of aorta samples (n = 49) were used for dual-tracer autoradiography, and regional tracer uptake levels were evaluated. Lesions were identified histologically with Movat's pentachrome (AHA lesion phenotypes), Mac-2 staining (macrophage infiltration) and Oil Red O staining (lipid deposition).

Results

The highest uptakes of [14C]FDG (3.10 ± 1.50 %ID × kilogram per square millimeter) and [99mTc]annexin A5 (0.49 ± 0.20 %ID × kilogram per square millimeter) were shown in atheromatous lesions (types III and IV). Each tracer uptake showed better correlation with macrophage infiltration than lipid deposition ([14C]FDG, r = 0.44 vs. r = 0.14; [99mTc]annexin A5, r = 0.65 vs. r = 0.48).

Conclusions

Both tracers were concentrated in type III and IV atheromatous lesions which corresponded to macrophage infiltration rather than lipid deposition.

Key words

AtherosclerosisImagingNuclear medicineFDGAnnexin A5

Abbreviations

FDG

2-Deoxy-2-fluoro-D-glucose

HYNIC

Hydrazinonicotinamide

[99mTc]annexin A5

[99mTc]HYNIC-annexin A5

apoE−/− mice

Apolipoprotein E knockout mice

PET

Positron emission tomography

CT

Computed tomography

MRI

Magnetic resonance image

TIA

Transient ischemic attack

ARG

Autoradiography

ROI

Region of interest

ACS

Acute coronary syndrome

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010