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Simultaneous Dual-tracer PET Imaging of the Rat Brain and its Application in the Study of Cerebral Ischemia

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Abstract

Purpose

This study evaluates the performance of simultaneous dual-tracer technique (SDTT) in static positron emission tomography (PET) studies using 2-deoxy-2-[18F]fluoro-D-glucose and [13N]ammonium as radiotracers.

Procedures

The effects of applying SDTT either to the reconstructed image or directly to the sinogram, different rebinning algorithms, total acquisition time, and frame duration were investigated; first, using a specific phantom and later using an in vivo application of the study of cerebral ischemia.

Results

The best results were obtained using the image method with single-slice rebinning and a total acquisition time of at least 20 min. Frame duration did not affect SDTT performance. The method was also applied in rats with transient cerebral ischemia to simultaneously study cerebral blood flow and cerebral glucose metabolism.

Conclusion

The results encourage the use of SDTT as it has very good potential for examining two different biological processes at the same time utilising rodent PET scanners.

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Acknowledgements

This work was partially supported by the Ministerio de Industria, Turismo y Comercio (Cenit-Ingenio Program: CDTEAM project).

Francisca P. Figueiras has a Ph.D fellowship from the Fundação para a Ciência e a Tecnologia , POCI 2010, FSE SFRH/BD/38341/2007, and the European Community's Seventh Framework Program (FP7/2007-2013), HEALTH F2 2008 200728.

Disclosure/Conflict of Interest

The authors declare that they have no proprietary, financial, professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, this manuscript.

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Correspondence to Juan D. Gispert.

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Manuscript Category and its Significance: article—to our knowledge, this is the first report of simultaneous dual-isotope PET imaging in vivo, after determining the optimum parameters from phantom experiments. While some other articles study the applicability of this technique from the mathematical perspective or conducted phantom experiments solely, we demonstrate its applicability for the in vivo simultaneous determination of cerebral blood perfusion and glucose consumption.

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Figueiras, F.P., Jiménez, X., Pareto, D. et al. Simultaneous Dual-tracer PET Imaging of the Rat Brain and its Application in the Study of Cerebral Ischemia. Mol Imaging Biol 13, 500–510 (2011). https://doi.org/10.1007/s11307-010-0370-5

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