Molecular Imaging and Biology

, Volume 13, Issue 1, pp 78–86

Replacing Vascular Corrosion Casting by In Vivo Micro-CT Imaging for Building 3D Cardiovascular Models in Mice

  • Bert Vandeghinste
  • Bram Trachet
  • Marjolijn Renard
  • Christophe Casteleyn
  • Steven Staelens
  • Bart Loeys
  • Patrick Segers
  • Stefaan Vandenberghe
Research Article

DOI: 10.1007/s11307-010-0335-8

Cite this article as:
Vandeghinste, B., Trachet, B., Renard, M. et al. Mol Imaging Biol (2011) 13: 78. doi:10.1007/s11307-010-0335-8
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Abstract

Purpose

The purpose of this study was to investigate if in vivo micro-computed tomography (CT) is a reliable alternative to micro-CT scanning of a vascular corrosion cast. This would allow one to study the early development of cardiovascular diseases.

Procedures

Datasets using both modalities were acquired, segmented, and used to generate a 3D geometrical model from nine mice. As blood pool contrast agent, Fenestra VC-131 was used. Batson’s No. 17 was used as casting agent. Computational fluid dynamics simulations were performed on both datasets to quantify the difference in wall shear stress (WSS).

Results

Aortic arch diameters show 30% to 40% difference between the Fenestra VC-131 and the casted dataset. The aortic arch bifurcation angles show less than 20% difference between both datasets. Numerically computed WSS showed a 28% difference between both datasets.

Conclusions

Our results indicate that in vivo micro-CT imaging can provide an excellent alternative for vascular corrosion casting. This enables follow-up studies.

Key words

FenestraIn vivoMicro-CTMiceVascular corrosion casting3D modelWall shear stress

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010

Authors and Affiliations

  • Bert Vandeghinste
    • 1
  • Bram Trachet
    • 2
  • Marjolijn Renard
    • 3
  • Christophe Casteleyn
    • 4
  • Steven Staelens
    • 1
  • Bart Loeys
    • 3
  • Patrick Segers
    • 2
  • Stefaan Vandenberghe
    • 1
  1. 1.IBITECH-MEDISIPGhent University—IBBTGhentBelgium
  2. 2.IBITECH-bioMMedaGhent UniversityGhentBelgium
  3. 3.Centre for Medical GeneticsGhent UniversityGhentBelgium
  4. 4.Department of MorphologyGhent UniversityMerelbekeBelgium