Molecular Imaging and Biology

, Volume 13, Issue 1, pp 10–15

Reduced PBR/TSPO Expression After Minocycline Treatment in a Rat Model of Focal Cerebral Ischemia: A PET Study Using [18F]DPA-714

  • Abraham Martín
  • Raphaël Boisgard
  • Michael Kassiou
  • Frédéric Dollé
  • Bertrand Tavitian
Brief Article

DOI: 10.1007/s11307-010-0324-y

Cite this article as:
Martín, A., Boisgard, R., Kassiou, M. et al. Mol Imaging Biol (2011) 13: 10. doi:10.1007/s11307-010-0324-y

Abstract

Background

Many new candidate pharmaceuticals designed to improve recovery after stroke have been proposed recently, but there are still too few molecular imaging methods capable to assess their efficacy. A hallmark of the inflammatory reaction that follows focal cerebral ischemia is overexpression of the mitochondrial peripheral benzodiazepine receptor/18 kDa translocator protein (PBR/TSPO) in the monocytic lineage and astrocytes. This overexpression can be imaged with positron emission tomography (PET) using PBR/TSPO-selective radioligands such as [18F]DPA-714.

Purpose

Here, we tested whether PET with [18F]DPA-714 would evidence the effect of minocycline, a broad spectrum antibiotic presently tested as neuroprotective agent after stroke, on the inflammatory reaction induced in an experimental model of stroke.

Procedures

Ten rats were subjected to a 2-h transient middle cerebral artery occlusion with reperfusion. Minocycline or saline was intravenously administrated 1 h after reperfusion and daily during the following 6 days. PET studies were performed using [18F]DPA-714 at 7 days after cerebral ischemia.

Results

In vivo PET imaging showed a significant decrease in [18F]DPA-714 uptake at 7 days after cerebral ischemia in rats treated with minocycline with respect to saline-treated animals. Minocycline treatment had no effect on the size of the infarcted area.

Conclusion

Minocycline administered daily during 7 days after ischemia decreases [18F]DPA-714 binding, suggesting that the drug exerts an anti-inflammatory activity. [18F]DPA-714 PET is a useful biomarker to study novel anti-inflammatory strategies in experimental cerebral ischemia.

Key words

MinocyclinePETNeuroinflammationPBRTSPODPA-714Cerebral ischemia

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010

Authors and Affiliations

  • Abraham Martín
    • 1
  • Raphaël Boisgard
    • 1
  • Michael Kassiou
    • 2
  • Frédéric Dollé
    • 3
  • Bertrand Tavitian
    • 1
  1. 1.CEA, DSV, I²BM, SHFJ, Laboratoire Imagerie Moléculaire Expérimentale; INSERM U803Orsay CedexFrance
  2. 2.Department of Medical Radiation Sciences and School of ChemistryUniversity of SydneySydneyAustralia
  3. 3.CEA, DSV, I²BM, SHFJOrsayFrance