Fluorescence Imaging and Whole-Body Biodistribution of Near-Infrared-Emitting Quantum Dots after Subcutaneous Injection for Regional Lymph Node Mapping in Mice
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- Pic, E., Pons, T., Bezdetnaya, L. et al. Mol Imaging Biol (2010) 12: 394. doi:10.1007/s11307-009-0288-y
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This study compares fluorescence imaging to mass spectroscopy (inductively coupled plasma–mass spectroscopy, ICP–MS) for detection of quantum dots (QDs) in sentinel lymph node (LN) mapping of breast cancer.
We study the accumulation of near-infrared-emitting QDs into regional LNs and their whole-body biodistribution in mice after subcutaneous injection, using in vivo fluorescence imaging and ex vivo elemental analysis by ICP–MS.
We show that the QD accumulation in regional LNs is detectable by fluorescence imaging as early as 5 min post-delivery. Their concentration reaches a maximum at 4 h then decreases over a 10-day observation period. These data are confirmed by ICP–MS. The QD uptake in other organs, assessed by ICP–MS, increases steadily over time; however, its overall level remains rather low.
Fluorescence imaging can be used as a non-invasive alternative to ICP–MS to follow the QD accumulation kinetics into regional LNs.
Key wordsQuantum dots Lymph nodes Near-infrared fluorescence imaging Mass spectroscopy Biodistribution
- % ID
Percentage of injected dose
Axillary lymph node
Axillary lymph node dissection
Dynamic light scattering
Hematoxylin and eosin
Inductively coupled plasma–atomic emission spectroscopy
Inductively coupled plasma–mass spectroscopy
Left axillary lymph node
Left lateral thoracic lymph node
Lateral thoracic lymph node
Micro-positron emission tomography
Phosphate buffered saline
Right axillary lymph node
Right lateral thoracic lymph node
Region of interest
Sentinel lymph node
Sentinel lymph node biopsy
Transmission electron microscopy