Research Article

Molecular Imaging and Biology

, Volume 12, Issue 3, pp 325-334

First online:

Antioxidants Improve Early Survival of Cardiomyoblasts After Transplantation to the Myocardium

  • Martin Rodriguez-PorcelAffiliated withDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine Email author 
  • , Olivier GheysensAffiliated withDepartment of Nuclear Medicine, University Hospital Leuven
  • , Ramasamy PaulmuruganAffiliated withMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Division of Nuclear Medicine, Stanford University
  • , Ian Y. ChenAffiliated withMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Division of Nuclear Medicine, Stanford UniversityDepartment of Bioengineering, Stanford University
  • , Karen M. PetersonAffiliated withDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine
  • , Jürgen K. WillmannAffiliated withMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Division of Nuclear Medicine, Stanford University
  • , Joseph C. WuAffiliated withMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Division of Nuclear Medicine, Stanford University
  • , Xiangyang ZhuAffiliated withDivision of Nephrology and Hypertension, Department of Internal Medicine, Mayo Clinic College of Medicine
  • , Lilach O. LermanAffiliated withDivision of Nephrology and Hypertension, Department of Internal Medicine, Mayo Clinic College of Medicine
    • , Sanjiv S. GambhirAffiliated withMolecular Imaging Program at Stanford (MIPS), Department of Radiology, Division of Nuclear Medicine, Stanford UniversityDepartment of Bioengineering, Stanford University

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Abstract

Purpose

We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium.

Procedures

Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI.

Results

Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals.

Conclusion

Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.

Key words

Bioluminescence Firefly luciferase Myoblasts Molecular imaging Stem cell Oxidative stress Myocardium Antioxidants