Bioluminescence Imaging in Mouse Models Quantifies β Cell Mass in the Pancreas and After Islet Transplantation
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- Virostko, J., Radhika, A., Poffenberger, G. et al. Mol Imaging Biol (2010) 12: 42. doi:10.1007/s11307-009-0240-1
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We developed a mouse model that enables non-invasive assessment of changes in β cell mass.
We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased β cell mass and after islet transplantation.
Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in β cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in β cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year.
Bioluminescence in the MIP-Luc-VU mouse model is proportional to β cell mass in the setting of increased and decreased β cell mass and after transplantation.
Key wordsBioluminescence imaging Luciferase Beta cell Pancreatic islet Transplantation Optical imaging Islet mass Beta cell mass Imaging
Mouse insulin promoter
Mouse insulin promoter-luciferase-Vanderbilt University
Magnetic resonance imaging
Positron emission tomography
Pancreatic-duodenal homeobox factor-1
non-obese diabetic-severe combined immunodeficiency
Friend leukemia Virus B strain
Isobutyl methyl xanthine
Region of interest
Fetal bovine serum