Molecular Imaging and Biology

, Volume 11, Issue 3, pp 178–187

Comparison of Optical Bioluminescence Reporter Gene and Superparamagnetic Iron Oxide MR Contrast Agent as Cell Markers for Noninvasive Imaging of Cardiac Cell Transplantation

Authors

  • Ian Y. Chen
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Bio-X ProgramStanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of BioengineeringStanford University School of Medicine
  • Joan M. Greve
    • Department of BioengineeringStanford University School of Medicine
  • Olivier Gheysens
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Bio-X ProgramStanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of BioengineeringStanford University School of Medicine
  • Jürgen K. Willmann
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Bio-X ProgramStanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of BioengineeringStanford University School of Medicine
  • Martin Rodriguez-Porcel
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Bio-X ProgramStanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of BioengineeringStanford University School of Medicine
  • Pauline Chu
    • Department of Comparative MedicineStanford University School of Medicine
  • Ahmad Y. Sheikh
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Department of CardiothoracicStanford University School of Medicine
  • Anthony Z. Faranesh
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
  • Ramasamy Paulmurugan
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Bio-X ProgramStanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of BioengineeringStanford University School of Medicine
  • Phillip C. Yang
    • Department of Medicine, Division of Cardiovascular MedicineStanford University School of Medicine
  • Joseph C. Wu
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of Medicine, Division of Cardiovascular MedicineStanford University School of Medicine
    • Molecular Imaging Program at Stanford (MIPS)Stanford University School of Medicine
    • Bio-X ProgramStanford University School of Medicine
    • Department of RadiologyStanford University School of Medicine
    • Department of BioengineeringStanford University School of Medicine
Research Article

DOI: 10.1007/s11307-008-0182-z

Cite this article as:
Chen, I.Y., Greve, J.M., Gheysens, O. et al. Mol Imaging Biol (2009) 11: 178. doi:10.1007/s11307-008-0182-z

Abstract

Purpose

In this study, we compared firefly luciferase (Fluc) reporter gene and superparamagnetic iron oxide (Feridex) as cell markers for longitudinal monitoring of cardiomyoblast graft survival using optical bioluminescence imaging (BLI) and magnetic resonance imaging (MRI), respectively.

Procedures

Rats (n = 31) underwent an intramyocardial injection of cardiomyoblasts (2 × 106) labeled with Fluc, Feridex, or no marker (control) or an injection of Feridex alone (75 μg). Afterward, rats were serially imaged with BLI or MRI and killed at different time points for histological analysis.

Results

BLI revealed a drastically different cell survival kinetics (half-life = 2.65 days over 6 days) than that revealed by MRI (half-life = 16.8 days over 80 days). Injection of Feridex alone led to prolonged tissue retention of Feridex (≥16 days) and persistent MR signal (≥42 days).

Conclusions

Fluc BLI reporter gene imaging is a more accurate gauge of transplanted cell survival as compared to MRI of Feridex-labeled cells.

Key words

Optical bioluminescence imagingMagnetic resonance imagingReporter geneContrast agentCell markerCell transplantation

Copyright information

© Academy of Molecular Imaging 2008