Molecular Imaging and Biology

, Volume 8, Issue 5, pp 292–299

Evaluation of the Integrity of the Dopamine System in a Rodent Model of Parkinson’s Disease: Small Animal Positron Emission Tomography Compared to Behavioral Assessment and Autoradiography

  • Elissa M. Strome
  • Ivan L. Cepeda
  • Vesna Sossi
  • Doris J. Doudet
Research Article

DOI: 10.1007/s11307-006-0051-6

Cite this article as:
Strome, E.M., Cepeda, I.L., Sossi, V. et al. Mol Imaging Biol (2006) 8: 292. doi:10.1007/s11307-006-0051-6

Abstract

Purpose

In the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD), it is important to determine lesion severity. This evaluation can be performed in vivo, through evaluation of dopamine (DA)-dependent motor function or with small animal positron emission tomography (microPET), or at postmortem, by examining markers for DA neurons.

Procedures

Rats were given mild or severe unilateral 6-OHDA lesions, scanned with the tracer [11C](±)dihydrotetrabenazine ([11C]DTBZ), and tested on a tapered/ledged beam-walking task. At postmortem, autoradiography was performed with [11C]DTBZ.

Results

Autoradiography was significantly correlated with microPET and behavioral scores, whereas the microPET and behavioral data were not significantly correlated.

Conclusions

This study shows that behavioral analysis, microPET, and autoradiography are all good tools for measuring the integrity of the DA system, and demonstrates the utility of the tapered/ledged beam-walking test to screen for lesion severity, as well as the importance of including postmortem analysis after in vivo imaging studies.

Key words

MicroPET Rodent VMAT2 [11C]DTBZ Dopamine Striatum Partial volume effect 6-OHDA Behavior Tapered beam-walking task 

Copyright information

© Academy of Molecular Imaging 2006

Authors and Affiliations

  • Elissa M. Strome
    • 1
  • Ivan L. Cepeda
    • 1
  • Vesna Sossi
    • 1
  • Doris J. Doudet
    • 1
  1. 1.Pacific Parkinson’s Research CentreUniversity of British ColumbiaVancouverCanada