Metabolomics

, Volume 11, Issue 2, pp 380–390

Anti-malarial drug artesunate restores metabolic changes in experimental allergic asthma

  • Wanxing Eugene Ho
  • Yong-Jiang Xu
  • Fengguo Xu
  • Chang Cheng
  • Hong Yong Peh
  • Shao-Min Huang
  • Steven R. Tannenbaum
  • Choon Nam Ong
  • W. S. Fred Wong
Original Article

DOI: 10.1007/s11306-014-0699-x

Cite this article as:
Ho, W.E., Xu, Y., Xu, F. et al. Metabolomics (2015) 11: 380. doi:10.1007/s11306-014-0699-x

Abstract

The anti-malarial drug artesunate possesses anti-inflammatory and anti-oxidative actions in experimental asthma, comparable to corticosteroid. We hypothesized that artesunate may modulate disease-relevant metabolic alterations in allergic asthma. To explore metabolic profile changes induced by artesunate in allergic airway inflammation, we analysed bronchoalveolar lavage fluid (BALF) and serum from naïve and ovalbumin-induced asthma mice treated with artesunate, using both gas and liquid chromatography-mass spectrometry metabolomics. Pharmacokinetics analyses of serum and lung tissues revealed that artesunate is rapidly converted into the active metabolite dihydroartemisinin. Artesunate effectively suppressed BALF total and differential counts, and repressed BALF Th2 cytokines, IL-17, IL-12(p40), MCP-1 and G-CSF levels. Artesunate had no effects on both BALF and serum metabolome in naïve mice. Artesunate promoted restoration of BALF sterols (cholesterol, cholic acid and cortol), phosphatidylcholines and carbohydrates (arabinose, mannose and galactose) and of serum 18-oxocortisol, galactose, glucose and glucouronic acid in asthma. Artesunate prevented OVA-induced increases in pro-inflammatory metabolites from arginine–proline metabolic pathway, particularly BALF levels of urea and alanine and serum levels of urea, proline, valine and homoserine. Multiple statistical correlation analyses revealed association between altered BALF and serum metabolites and inflammatory cytokines. Dexamethasone failed to reduce urea level and caused widespread changes in metabolites irrelevant to asthma development. Here we report the first metabolome profile of artesunate treatment in experimental asthma. Artesunate restored specific metabolic perturbations in airway inflammation, which correlated well with its anti-inflammatory actions. Our metabolomics findings further strengthen the therapeutic value of using artesunate to treat allergic asthma.

Keywords

MetabolomeArtemisininsMass spectrometryAllergic asthmaCorticosteroid

Supplementary material

11306_2014_699_MOESM1_ESM.docx (425 kb)
Supplementary material 1 (DOCX 425 kb)

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Wanxing Eugene Ho
    • 1
    • 2
  • Yong-Jiang Xu
    • 1
    • 3
  • Fengguo Xu
    • 1
    • 4
  • Chang Cheng
    • 5
  • Hong Yong Peh
    • 5
  • Shao-Min Huang
    • 1
  • Steven R. Tannenbaum
    • 2
    • 6
  • Choon Nam Ong
    • 1
    • 7
  • W. S. Fred Wong
    • 5
    • 8
  1. 1.Saw Swee Hock School of Public Health, National University Health SystemNational University of SingaporeSingaporeSingapore
  2. 2.Singapore-MIT Alliance for Research and Technology (SMART)SingaporeSingapore
  3. 3.Key Laboratory of Insect Development and Evolutionary BiologyChinese Academy of SciencesShanghaiChina
  4. 4.Key Laboratory of Drug Quality Control and PharmacovigilanceChina Pharmaceutical UniversityNanjingChina
  5. 5.Department of Pharmacology, Yong Loo Lin School of MedicineNational University Health SystemSingaporeSingapore
  6. 6.Department of Biological Engineering and ChemistryMassachusetts Institute of TechnologyCambridgeUSA
  7. 7.NUS Environmental Research InstituteNational University of SingaporeSingaporeSingapore
  8. 8.Immunology Program, Life Science InstituteNational University of SingaporeSingaporeSingapore