Metabolomics

, Volume 11, Issue 2, pp 251–260

Metabolite profiling of Clostridium difficile ribotypes using small molecular weight volatile organic compounds

  • S. Kuppusami
  • M. R. J. Clokie
  • T. Panayi
  • A. M. Ellis
  • P. S. Monks
Original Article

DOI: 10.1007/s11306-014-0692-4

Cite this article as:
Kuppusami, S., Clokie, M.R.J., Panayi, T. et al. Metabolomics (2015) 11: 251. doi:10.1007/s11306-014-0692-4

Abstract

Volatile organic compounds (VOCs) emitted by cultures of ten different Clostridium difficile ribotypes have been profiled using proton transfer reaction-time of flight-mass spectrometry. A total of 69 VOCs were identified and combinations of these VOCs were found to be characteristic for each of the ribotypes. The VOC patterns, with the aid of a statistical analysis, have been shown to be useful in distinguishing different ribotypes. A tentative assignment of different masses also shows that different ribotypes have markedly different emissions of methanol, p-cresol, dimethylamine and a range sulfur compounds (ethylene sulfide, dimethylsulfide and methyl thioacetate), which point to VOCs as potential indicators of different metabolic pathways in virulent and less-virulent strains. The results establish the potential of detecting emitted VOC metabolites to differentiate between closely related C. difficile ribotypes and in the longer term provide metabolic insight into virulence.

Keywords

Volatile organic compoundsClostridium difficileRibotype

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • S. Kuppusami
    • 1
  • M. R. J. Clokie
    • 2
  • T. Panayi
    • 2
  • A. M. Ellis
    • 1
  • P. S. Monks
    • 1
  1. 1.Department of ChemistryUniversity of LeicesterLeicesterUK
  2. 2.Department of Immunity, Infection and InflammationUniversity of LeicesterLeicesterUK