Original Article

Metabolomics

, Volume 9, Issue 3, pp 599-607

Open Access This content is freely available online to anyone, anywhere at any time.

Phytochemical composition of Potentilla anserina L. analyzed by an integrative GC-MS and LC-MS metabolomics platform

  • Angela MariAffiliated withDepartment of Pharmaceutical and Biomedical Sciences, University of Salerno
  • , David LyonAffiliated withDepartment of Molecular Systems Biology, University of Vienna
  • , Lena FragnerAffiliated withDepartment of Molecular Systems Biology, University of Vienna
  • , Paola MontoroAffiliated withDepartment of Pharmaceutical and Biomedical Sciences, University of Salerno
  • , Sonia PiacenteAffiliated withDepartment of Pharmaceutical and Biomedical Sciences, University of Salerno Email author 
  • , Stefanie WienkoopAffiliated withDepartment of Molecular Systems Biology, University of Vienna
  • , Volker EgelhoferAffiliated withDepartment of Molecular Systems Biology, University of Vienna
  • , Wolfram WeckwerthAffiliated withDepartment of Molecular Systems Biology, University of Vienna Email author 

Abstract

Potentilla anserina L. (Rosaceae) is known for its beneficial effects of prevention of pre-menstrual syndrome (PMS). For this reason P. anserina is processed into many food supplements and pharmaceutical preparations. Here we analyzed hydroalcoholic reference extracts and compared them with various extracts of different pharmacies using an integrative metabolomics platform comprising GC-MS and LC-MS analysis and software toolboxes for data alignment (MetMAX Beta 1.0) and multivariate statistical analysis (COVAIN 1.0). Multivariate statistics of the integrated GC-MS and LC-MS data showed strong differences between the different plant extract formulations. Different groups of compounds such as chlorogenic acid, kaempferol 3-O-rutinoside, acacetin 7-O-rutinoside, and genistein were reported for the first time in this species. The typical fragmentation pathway of the isoflavone genistein confirmed the identification of this active compound that was present with different abundances in all the extracts analyzed. As a result we have revealed that different extraction procedures from different vendors produce different chemical compositions, e.g. different genistein concentrations. Consequently, the treatment may have different effects. The integrative metabolomics platform provides the highest resolution of the phytochemical composition and a mean to define subtle differences in plant extract formulations.

Keywords

Medicinal plants Metabolomics GC-MS LC-MS Flavonoids Genistein Mass accuracy precursor alignment (MAPA)