Metabolomics

, Volume 8, Issue 3, pp 465–474

Metabonomic analysis of saliva reveals generalized chronic periodontitis signature

  • Mario Aimetti
  • Stefano Cacciatore
  • Antonio Graziano
  • Leonardo Tenori
Original Article

DOI: 10.1007/s11306-011-0331-2

Cite this article as:
Aimetti, M., Cacciatore, S., Graziano, A. et al. Metabolomics (2012) 8: 465. doi:10.1007/s11306-011-0331-2

Abstract

The diagnoses of periodontal diseases (PD) are primarily based on clinical examination and radiographic parameters. In this pilot exploration we want to supply some evidence whether metabonomic profiling of saliva samples can provide a signature of the disease. Saliva samples were analyzed by Nuclear Magnetic Resonance (NMR) metabonomics from 22 healthy subjects (HS) and 32 patients with clinic and radiographic diagnosis of different PD: Gingivitis (G), Localized Chronic Periodontitis (LCP), Generalized Chronic Periodontitis (GCP), Localized Aggressive Periodontitis (LAP), and Generalized Aggressive Periodontitis (GAP). Pattern recognition analysis of NMR profiles can discriminate GCP patients (n = 21) from HS (n = 22) with an accuracy of 84.1%. Metabolic profiles of GCP patients exhibited higher concentrations of acetate, γ-aminobutyrate, n-butyrate, succinate, trimethylamine, propionate, phenylalanine and valine, and decreased concentrations of pyruvate and N-acetyl groups compared with controls. Our results can provide a contribution to the understanding of the biochemical network and pathway in the GCP and other PD, however at this stage the method can not be extended to the general population as a ready-to-use clinical tool, due to the limited cohort recruited and the exploratory nature of this work. Anyway, a further validation of the statistical model on a larger cohort is in progress with the aim to demonstrate the potential impact in clinical practice of our findings.

Keywords

Metabolomics Metabonomics NMR Saliva Periodontal diseases Support vector machine Periodontitis 

Supplementary material

11306_2011_331_MOESM1_ESM.doc (453 kb)
Supplementary material 1 (DOC 453 kb)

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mario Aimetti
    • 3
  • Stefano Cacciatore
    • 1
    • 2
  • Antonio Graziano
    • 3
    • 4
  • Leonardo Tenori
    • 2
  1. 1.CERM and Department of ChemistryUniversity of FlorenceSesto FiorentinoItaly
  2. 2.FiorGen FoundationSesto FiorentinoItaly
  3. 3.Department of PeriodontologyUniversity of Turin Dental SchoolTurinItaly
  4. 4.Department of BiotechnologyCST, SHRO, Temple UniversityPhiladelphiaUSA

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