Metabolomics

, Volume 7, Issue 3, pp 375–387

Metabolomics of prolonged fasting in humans reveals new catabolic markers

Authors

  • Isabel Rubio-Aliaga
    • Molecular Nutrition Unit, ZIEL—Research Center for Nutrition and Food SciencesTechnische Universität München
  • Baukje de Roos
    • Rowett Institute of Nutrition and HealthUniversity of Aberdeen
  • Susan J. Duthie
    • Rowett Institute of Nutrition and HealthUniversity of Aberdeen
  • L. Katie Crosley
    • Rowett Institute of Nutrition and HealthUniversity of Aberdeen
  • Claus Mayer
    • Biomathematics and Statistics Scotland
  • Graham Horgan
    • Biomathematics and Statistics Scotland
  • Ian J. Colquhoun
    • Institute of Food Research
  • Gwénaëlle Le Gall
    • Institute of Food Research
  • Fritz Huber
    • LIPOFIT Analytics GmbH
  • Werner Kremer
    • LIPOFIT Analytics GmbH
  • Michael Rychlik
    • Bioanalytik Weihenstephan, ZIEL—Research Center for Nutrition and Food SciencesTechnische Universität München
  • Suzan Wopereis
    • Physiological GenomicsTNO-Quality of Life
  • Ben van Ommen
    • Physiological GenomicsTNO-Quality of Life
  • Gabriele Schmidt
    • Molecular Nutrition Unit, ZIEL—Research Center for Nutrition and Food SciencesTechnische Universität München
  • Carolin Heim
    • Molecular Nutrition Unit, ZIEL—Research Center for Nutrition and Food SciencesTechnische Universität München
  • Freek G. Bouwman
    • Functional GeneticsMaastricht University
  • Edwin C. Mariman
    • Functional GeneticsMaastricht University
  • Francis Mulholland
    • Institute of Food Research
  • Ian T. Johnson
    • Institute of Food Research
  • Abigael C. Polley
    • Institute of Food Research
  • Ruan M. Elliott
    • Institute of Food Research
    • Molecular Nutrition Unit, ZIEL—Research Center for Nutrition and Food SciencesTechnische Universität München
Original Article

DOI: 10.1007/s11306-010-0255-2

Cite this article as:
Rubio-Aliaga, I., de Roos, B., Duthie, S.J. et al. Metabolomics (2011) 7: 375. doi:10.1007/s11306-010-0255-2

Abstract

Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human “fasting metabolome” obtained from analysis of plasma and urine samples in a small cohort of healthy volunteers, using nuclear magnetic resonance (NMR), gas chromatography- and liquid chromatography-mass spectrometry (GC-MS and LC-MS). Intra- and inter-individual variation of metabolites was on measurement of four overnight fasting samples collected from each volunteer over a four week period. One additional sample per volunteer was collected following a prolonged fasting period of 36 h. Amongst a total of 377 quantified entities in plasma around 44% were shown to change significantly in concentration when volunteers extended fasting from 12 to 36 h. In addition to known markers (plasma free fatty acids, glycerol, ketone bodies) that reflect changes in the body’s fuel management under fasting conditions a wide range of “new” entities such as α-aminobutyrate as well as other amino and keto acids were identified as fasting markers. Based on multiple correlations amongst the metabolites and selected hormones in plasma such as leptin or insulin-like-growth-factor-1 (IGF-1), a robust metabolic network with coherent regulation of a wide range of metabolites could be identified. The metabolomics approach described here demonstrates the plasticity of human metabolism and identifies new and robust markers of the fasting state.

Keywords

Fasting responseMetabolite profilingSubject variability

Supplementary material

11306_2010_255_MOESM1_ESM.pdf (860 kb)
Supplementary material 1 (PDF 861 kb)
11306_2010_255_MOESM2_ESM.doc (38 kb)
Supplementary material 2 (DOC 39 kb)
11306_2010_255_MOESM3_ESM.xls (82 kb)
Supplementary material 3 (XLS 83 kb)

Copyright information

© Springer Science+Business Media, LLC 2010