Metabolomics

, Volume 7, Issue 2, pp 226–236

Metabolic profiling reveals the protective effect of diammonium glycyrrhizinate on acute hepatic injury induced by carbon tetrachloride

  • Xiaoyan Wang
  • Jingchao Lin
  • Tianlu Chen
  • Mingmei Zhou
  • Mingming Su
  • Wei Jia
Original Article

DOI: 10.1007/s11306-010-0244-5

Cite this article as:
Wang, X., Lin, J., Chen, T. et al. Metabolomics (2011) 7: 226. doi:10.1007/s11306-010-0244-5

Abstract

Diammonium glycyrrhizinate (DG), a constitutent of Glycyrrhiza uralensis, has a protective effect on hepatic injury, hepatisis and cirrhosis. To date, the mechanism has been poorly understood, especially at the metabolic level. A metabolomic profiling study was performed to characterize the carbon tetrachloride (CCl4) induced global metabolic alteration and the protective effects of DG in Sprague-Dawley rats. Urinary and hepatic tissue metabolic profiling revealed that CCl4 perturbed the amino acid metabolism (alanine, glycine, leucine), tricarboxylic acid cycle (citrate), lipid metabolism (unsaturated fatty acids) and gut microbiota related metabolites. Our results also indicated that DG was able to attenuate CCl4 perturbed metabolic pathways and ameliorated biochemical markers of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Total cholesterol (TCHO). This global metabolomic approach also revealed full metabolic recovery takes longer than apparent and conventional histological and biochemical markers.

Keywords

Metabolomics Acute liver injure Diammonium glycyrrhizinate Carbon tetrachloride 

Abbreviations

DG

Diammonium glycyrrhizinate

NC

Normal control

TCHO

Total cholesterol

AST

Aspartate aminotransferase

ALT

Alanine aminotransferase

MDA

Malondiadehyde

SOD

Superoxide dismutase

GSH-px

Glutathion peroxidase

ECF

Ethylchloroformate

PCA

Principal component analysis

PLS-DA

Partial least squares discriminant analysis

PC

Principal component

GSH

Reduced glutathione

GSSG

Oxidized glutathione

DOPAC

3,4-Dihydroxy-phenylacetate

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Xiaoyan Wang
    • 1
  • Jingchao Lin
    • 1
  • Tianlu Chen
    • 1
  • Mingmei Zhou
    • 2
  • Mingming Su
    • 3
  • Wei Jia
    • 3
    • 4
  1. 1.Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems BiomedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Shanghai University of Traditional Chinese MedicineShanghaiChina
  3. 3.David H. Murdock Research InstituteUniversity of North Carolina at Greensboro, North Carolina Research CampusKannapolisUSA
  4. 4.Department of NutritionUniversity of North Carolina at Greensboro, North Carolina Research CampusKannapolisUSA