ω-3 oil intake during weight loss in obese women results in remodelling of plasma triglyceride and fatty acids
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- McCombie, G., Browning, L.M., Titman, C.M. et al. Metabolomics (2009) 5: 363. doi:10.1007/s11306-009-0161-7
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Previous studies have shown that a combination of weight loss and fish oil supplementation reduce cardiovascular disease and diabetes risks by increasing adiponectin and reducing triacylglyceride concentrations, while weight loss alone significantly improves insulin sensitivity and reduces inflammation. Here, a metabolomic approach, using a combination of 1H-Nuclear Magnetic Resonance spectroscopy, and gas and liquid chromatography and mass spectrometry, was employed to elucidate the metabolic changes in blood plasma following weight loss and fish oil supplementation. The intervention study was conducted over 24 weeks, with 93 female subjects randomised to one of three groups. Two groups followed a 12-week weight loss program, followed by a 12-week weight maintenance period and were randomised to fish or placebo oil capsules; a control group did not follow the weight loss program and were given placebo oil capsules. Lipid profiles changed dramatically upon fish oil intake and subtly across the two weight loss groups. While the fish oil supplementation increased the proportion of various phospholipid species, previously reported reductions in total triacylglycerides (TAGs) upon fish oil intake were shown to be driven by a reduction in a specific subset of the measured TAGs. This remodelling of triglycerides may represent further beneficial effects of fish oil supplementation.
KeywordsMetabolomicsMetabonomics weight lossOmega-3 polyunsaturated fatty acidsPUFANMRLC-MSGC-MSTriacylglycerol
Obesity is one of the major epidemics in western societies. In the USA obesity has doubled in adults and overweight prevalence tripled in children and adolescents from 1980 to 2002 (Ogden et al. 2006). Obesity is correlated with a higher risk of premature death (Manson et al. 1995), and in particular increased risk in the development of diabetes (Chan et al. 1994; Colditz et al. 1990), hypertension (Stamler et al. 1978) and cardiovascular disease (CVD) (Manson et al. 1990). Weight loss has proven to significantly improve insulin sensitivity, reduce blood pressure, and lower the risks associated with CVD risk and type 2 diabetes. In two independent large scale intervention programs, a weight loss of 6% as part of a lifestyle modification programme reduced the risk of progression from impaired glucose tolerance to type 2 diabetes by almost 60% (Knowler et al. 2002; Tuomilehto et al. 2001).
Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA), either as a supplement or when consumed as oily fish, have many beneficial effects on various measures associated with obesity and pathologies associated with the metabolic syndrome. It has been well established that fish oil intake lowers the total triacylglycerol concentration considerably more than weight loss does (Harris 1996). Furthermore, it has been suggested that dietary fish oil supplements protect against weight gain in animals (Kriketos et al. 2001). Fish oil has an anti-inflammatory effect, as the LC n-3 PUFA have a lower inflammatory response than the equivalent LC n-6 PUFA, even though both n-3 and n-6 are precursors for inflammatory eicosanoids (James et al. 2000), providing an additional protective effect against CVD and diabetes, independent of BMI (Browning et al. 2004; Hill et al. 2007).
The aim of this study was to examine synergistic effects on CVD risk factors when combining weight loss with fish oil intake. We have already reported that in this study the weight loss intervention improved insulin sensitivity, reduced plasma triacylglycerides and overall inflammation, with additional decreases in blood plasma triglycerides and increased adiponectin associated with fish oil supplementation (Krebs et al. 2006). In the present analysis we have used a combined metabolomic approach to further characterise the metabolic changes that accompany combined weight loss and fish oil supplementation to better understand how these interventions produce a beneficial effect in obese individuals. Metabolomics is the study of all small molecule metabolites in a cell, tissue, organ or organism and the changes thereof due to genetic modification, disease or environmental stimuli (Weckwerth 2003; Griffin 2004). Analysis of the changing metabolite concentrations can then show which biological processes are involved following a given biological stimulus. Many of the mechanisms by which health benefits arise from weight loss or fish oil intake are yet unclear on a metabolic level. In this study we used 1H Nuclear Magnetic Resonance (NMR) spectroscopy, gas and liquid chromatography and mass spectrometry (GC-MS and LC-MS) to investigate the changes in metabolism upon fish oil intake and weight loss.
2 Materials and methods
2.1 Study design
Summary of selected clinical data (SD) for the subject groups studied. For more information see (Krebs et al. 2006)
n = 35
n = 35
n = 35
n = 32
n = 32
n = 32
n = 26
n = 26
n = 26
Waist circumfence (cm)
BMI (Kg m−2)
AUC insulin (pmol/120 min)
AUC glucose (mmol/120 min)
2.2 1H-NMR spectroscopy of blood plasma
Plasma samples (150 μl) were diluted with 450 μl of 0.9% NaCl in 2H2O (w/v). Spectra were acquired using a 5 mm inverse-detection TXI probe in an 11.7 Tesla magnet (500.3 MHz proton frequency) interfaced to a Bruker AVANCE II+ spectrometer (Bruker, Coventry, UK), allowing the simultaneous measurement of ~30 metabolites which can be identified by comparison with reference spectra. Spectra were acquired at 30°C and were the sum of 64 transients acquired into 32 K data points using the 1-D version of the noesypr1d pulse sequence: Relaxation Delay (RD)-90°-t1-90°-tm-90°-acquire; RD = 1.5 s, t1 = 4 μs; TR = 2 s. The sweep width was 8,000 Hz and the water resonance was suppressed by low power irradiation during the mixing time (tm) of 50 ms and the relaxation delay. Spectra were Fourier transformed following multiplication by a 0.3 Hz exponential function.
Spectra were processed (phased and baseline corrected) using the ADC/Spec Manager (Version 9.15) software. The phase and the baseline of the spectra were manually corrected. Integration of peaks was processed for regions 0.20–4.20 ppm and 5.08–9.60 ppm, excluding the water resonance, with an integral width of 0.04 ppm, producing 213 integral buckets in total. All spectra were normalised to total signal. The data were analysed using principal components analysis (PCA) and partial least square discriminant analysis (PLS-DA) (see below).
2.3 Blood plasma lipid extraction
Metabolite extraction was achieved using methanol, chloroform and water based on the method of Bligh and Dyer (Bligh and Dyer 1959). Briefly, 150 μl of blood plasma were mixed with 600 μl methanol: chloroform (2:1). Samples were sonicated for 15 min. Water and chloroform were added (200 μl of each) and the samples centrifuged for 20 min. The resulting aqueous and organic layers were separated from the protein pellet. The organic layer was dried overnight in a fume food whilst the aqueous extracts were dried using an evacuated centrifuge (Eppendorf, Hamburg, Germany).
2.4 1H-NMR spectroscopy of aqueous blood plasma extracts
The dried extracts of the aqueous phase were rehydrated in 600 μl of D2O, buffered with 15 mM sodium phosphate (pH 7.4) containing 1 mM sodium-3-(tri-methylsilyl)-2,2,3,3-tetradeuterio-propionate (TSP; Cambridge Isotope Laboratories, Andover, MA) as an internal standard. The samples were analysed using the pulse sequence, NMR spectrometer, magnet and probe described above. Spectra were collected across 128 transients into 32 k data points and with a 2 s inter-pulse relaxation delay. The spectra were processed using the method described above for blood plasma.
2.5 Gas chromatography—mass spectrometry (GC-MS)
Lipids were extracted from the plasma sample using a Folch extraction to prepare fatty acid methyl esters (Folch et al. 1957). One millilitre of methanol, containing 100 μg/ml undecanoic acid (C11:0), was added to 200 μl sample. The contents were vortex mixed for 1 min at 1,000 rpm, before adding 2 ml of chloroform. The contents were mixed, as before, for a further minute. 1 ml of 2% potassium chloride was added and the contents were mixed as previously described. The test-tube was transferred to a centrifuge for 5 min at 2,000 rpm at a temperature of 4°C. The lower chloroform layer was removed to a 12 × 75 mm glass tube and evaporated to near dryness in a vacuum concentrator at 45°C for 15 min. The residue was reconstituted in 3 ml of methanol saturated with sodium hydroxide (a concentration of ~1 mol/l.) The contents were transferred to a 25 ml tube, with stopper, and heated at 70°C in a water bath for 30 min. After a brief period of cooling, to condense toxic vapor, 4 ml of methanol containing 14% boron trifluoride was added, and the contents heated at 70°C for a further 20 min. 1 ml n-hexane was added to the tube to extract the derivatized fatty acids (Morrison and Smith 1964). Fifteen millilitres of de-ionized water was added to the test-tube, to stop the reaction. The test-tube was left for a short time to allow the contents to separate into discreet layers. The upper n-hexane layer was removed to a fresh 12 × 75 mm glass tube, containing a little anhydrous sodium sulphate, as drying agent.
Samples were then injected into a Shimadzu GC-17AAF gas chromatograph (Shimadzu Corporation, Kyoto, Japan), fitted with a SGE BPX70 column, (SGE International, Australia) using a programmed temperature vaporisation method. Samples were maintained at 55°C for 1 min, then increased to a temperature of 170°C at a rate of 14°C/min, then to 220°C at 3.5°C/min, where it was held for 1 min. A chromatogram was then obtained. Concentrations were calculated using the internal standard (C11:0) recovery and measured against a multi level calibration curve, plotted for each quantified fatty acid methyl ester using Shimadzu VP-software. The table containing the levels of 26 fatty acids for all samples was used to perform PCA and PLS-DA. As absolute values were used no normalisation was necessary.
2.6 Liquid chromatography—mass spectrometry (LC-MS)
Lipid extracts were re-dissolved in 2:1 methanol:chloroform and injected into a UPLC Acquity™ (Waters, Milford, USA) with a C8 column (ACQUITY UPLC™ BEH C8 2.1 × 100 mm, 1.7 μm, Waters). Mobile phase A was made up of water with 0.1% formic acid and 10 mM ammonium acetate, while phase B was acetonitrile:isopropanol (5:2) with 0.1% formic acid and 10 mM ammonium acetate. The analytes were eluted from the column over 10 min with a gradient of 60–100% B followed by 2 min at 100% B and 2 min of column re-equilibration at the starting condition 60% B. The column flow rate was 600 μl/min. The UPLC was coupled to a Q-ToF micro (Waters, Milford, USA) with a 1:1 eluent split. After every 10 sample injections a pooled sample followed by a blank were injected in order to ensure consistent performance of the system. The randomised sample list of extracts was run three times, resulting in the repeating measurements being a few days apart. MassLynx (Waters, Milford, USA) was used to export spectra as ASCII files and further processing was done using in-house software written in the package IGOR PRO (WaveMetrics, Lake Oswego, Oregon, USA). Every LC-MS file was compressed to the mass spectral dimension by adding all retention time points at a given m/z value. The resulting, summed mass spectra were aligned to correct for mass drifts over the days of the analysis. Every data point in the spectrum was kept as a variable in the following SIMCA-P + 11 (v11.0, Umetrics, Umea, Sweden) analysis if it exceeded the noise threshold in more than 10% of all the spectra from all samples. This left 1,745 data points for each spectrum to be analysed by PLS-DA. The spectral data for each sample was normalised to total ion count. Re-analysis of the raw data ensured that the extracted ion chromatograms of the significant loadings displayed only one peak and was not a combination of different compounds. Typically the average coefficient of variation for LC-MS measurements was ~10% of the mean as determined by repeated injections of the pool sample examined throughout the study. As expected, the values for specific lipids were below this value for high intensity peaks and higher for low abundant signals. Lipids were identified by a combination of retention time, mass and MS/MS measurements. No internal standard was used, as only relative changes were studied.
2.7 Multivariate data analysis of metabolomic data
For each analytical assay the resulting data table was imported into SIMCA-P (v11.0, Umetrics, Umea, Sweden) for multivariate analysis by PCA and PLS-DA (an extension of PCA used for classification). Data was normalised to total intensity (except GC-MS) and Pareto scaled. Identification of major metabolic perturbations within the pattern recognition models was achieved by analysis of corresponding loadings plots. The parameters R2 and Q2 were used as measures for the robustness of a pattern recognition model. R2 is the fraction of variance explained by a component, and cross validation of R2 gives Q2 which reveals the fraction of the total variation predicted by a component, it is a measure for the difference between two (or more) groups. Typically a robust model has R2 > 50% and Q2 > 40%.
For the LC-MS data the sections of the mass spectra that had been cut out for the analysis were filled with zero values in order to display the loadings resembling a differential mass spectrum for a more intuitive viewing. The peaks that contributed significantly to the separation as determined by the loading were then viewed as raw spectra in order to find the retention time of the mass that changed. Only in a few cases, were there more than one retention time for a specific ion, and even for these cases, the peak responsible for classification could be identified by visual inspection of raw chromatograms.
Independent of whether the samples were measured by NMR spectroscopy, GC-MS or LC-MS, the ability to distinguish between the groups shown in Fig. 1 followed the same general pattern. The biggest differences in the plasma profiles were associated with the intake of fish oil. The MS based methods, showed some more subtle changes associated with weight loss. The results obtained are discussed in detail below for the three analytical tools used.
3.1 High resolution 1H-NMR spectroscopy
As the lipid resonances overlap with aqueous metabolites, for example the 1.24–1.32 ppm region overlaps with a doublet from lactate, we examined aqueous extracts of the blood plasma to examine whether differences associated with aqueous metabolites and not lipid species were responsible for the classifications. However, analysis of the entire dataset demonstrated no separation for any of the groups, indicating that all classifications were associated with lipid species (data not shown). This conclusion held for all regions of the NMR spectrum where resonances from low molecular weight metabolites were obscured by broad lipid resonances. Thus, future analysis focussed on the lipid fraction.
3.2 GC-MS of total fatty acid content of blood plasma
As the NMR data suggested that the largest differences between the groups are associated with lipid moities, in the next stage of the analysis, the total fatty acid profiles were measured as fatty acid methyl esters (FAMEs) using GC-MS. This data has been previously presented using a univariate statistical approach (Krebs et al. 2006). Two way comparisons were made between the groups using pattern recognition methods. The most robust PLS-DA models of the data were built between IFO or FFO and any other group, indicating that fish oil supplementation dominated the measured changes. However, more subtle differences due to weight loss could be detected between IPO or IFO and IC. The robustness (Q2) was used to assess the validity of the models. While models with a Q2 > 0.08 are considered statistically significant, only those with Q2 > 0.4 robustly predict class membership for individual samples left out of the model. In these comparisons BFO/IFO had a Q2 of 0.63, while BPO/IPO had a Q2 of only 0.53, demonstrating that feeding fish oil had the biggest impact on the lipid profile while weight loss produced a less robust model.
Significant changes in FAMEs, with the intervention cause and direction of the changes
Change in dataset
Reduced with fish oil supplementation
Increased with fish oil supplementation
Reduced with weight loss at 12 & 24 weeks
Reduced with weight loss at 12 weeks
Increased with weight loss (in placebo group only)
From both visual inspection and pattern recognition analysis, as with the GC-MS data, the main differences in the lipid profile was due to the fish oil supplementation and only more subtle differences were found due to weight loss. A PLS-DA scores plot of all 3 groups at the intermediate time point show minor overlap between the IFO and IPO/IC groups and major overlap between the IPO and IC groups (supplementary Fig. 1).
The PLS-DA plots were validated by randomly permutating the value of Y and comparing the Q2 to the true value. In the case of the comparison between BC and IPO (Q2 = 0.5) the resulting regression line through the true and false Q2 s has an offset of −0.3 demonstrating the robustness of the model (supplementary Fig. 2). Similar results were achieved with all models considered significant (Q2 > 0.4) ensuring there was no over fitting of the data.
Summary of significant changes in LC-MS peaks between groups
Loading mass (M+H+)
Increased with fish oil supplementation
PS(36:6) or PC(36:5)
PS(36:6) or PC(36:5)
PS(40:7) or PC(40:6)
Decreased with fish oil supplementation
PS(32:2) or PC(32:1)a
Increased with weight loss (WLPO only)
Decreased with weight loss only
In terms of the class of lipid species affected the largest decreases on fish oil supplementation were associated with TAGs, suggesting that this class of lipids had decreased in general, which has been published before (Harris 1996; Krebs et al. 2006). This was confirmed by biochemical assay for total TAG concentration (e.g. P = 5.13 × 10−4 for BFO and IFO comparison, according to a Student t-test). However, some of the high intensity TAG signals, such as 52:3 and 52:2, in the raw data did not change significantly with fish oil intake indicating a remodelling of this class of lipid as well as an overall reduction. following fish oil feeding.
In this study we have employed a combined NMR spectroscopy, GC-MS and LC-MS metabolomic analysis of blood plasma in order to identify metabolic changes associated with fish oil supplementation, weight loss and the interaction between these two interventions. Currently no single analytical tool will provide a complete analysis of the metabolome and our strategy for open profiling is to use a combination of analytical devices. Although being the technique of least sensitivity, 1H-NMR spectroscopy was both rapid (~12 min per sample) and reproducible. However, because of the chemical nature of lipid species 1H-NMR spectroscopy provides poor discrimination between individual fatty acids. However, GC-MS provides a robust method for distinguishing changes in total fatty acid concentration in samples, but analysis times are longer (~30 min) and require prior derivatisation. LC-MS is capable of examining intact lipids directly. While assay times in this study were ~15 min per sample, the lower reproducibility of this approach compared with the other two analytical tools necessitated the analysis of samples in triplicate.
GC-MS is a robust method for fatty acid analysis and is often used for metabolomics. It does however, when applied to lipid analysis, only measure the total fatty acid composition. The information on the origin of the fatty acids is lost during the derivatisation process. LC-MS on the other hand measures intact lipid species but the information on the exact fatty acid composition is harder to determine. However, both techniques may be used complimentarily to maximise the information obtainable from the lipid fraction.
The data obtained by NMR spectroscopy demonstrated two important pieces of information that determined the direction of the mass spectrometric analyses. Firstly, no difference was found between samples for the aqueous metabolites but clear differences were detected for the whole plasma containing lipids. Secondly, the differences in the plasma lipids were, not surprisingly, associated with the degree of saturation in the fats of the groups supplementing their diet with fish oil. As there is significant resonance overlap between different fatty acids/lipid species further analyses were performed by mass spectrometry to determine the biochemical changes associated with fish oil supplementation.
The total reduction of TAGs upon increased intake of n-3 PUFAs that was found in these data is well established in the literature (Harris 1996; Krebs et al. 2006). However, we demonstrate in the present study that a remodelling of the TAG species has occurred. While the majority of the TAG(48:x where x signifies the number of double bonds) and TAG(50:x) decreased in concentration, the low abundance TAG(46:x) and TAG(56:x) and the high abundance TAG(52:x) and TAG(54:x) do not, with the exception of TAGs containing five unsaturated bonds across all three fatty acids.
The diverse range of changes detected in the lipid profile by LC-MS confirm previous literature reports where others have used LC-MS to study either animal models or patients with obesity, type 2 diabetes and the metabolic syndrome. Yetukuri and colleagues used LC-MS to study liver tissue of ob/ob mice and compared this to tissue from wild type animals (Yetukuri 2007). The authors found a general increase of short chain TAGs in ob/ob but not long chain ones. Specifically, TAG(48:2), TAG(48:1) and TAG(50:2) are decreased with fish oil supplementation in our study and increased in ob/ob mouse liver lipid profiles (Yetukuri 2007). Another study from the same group (Medina-Gomez 2007) identified a major decrease of TAG(48:2) in the POKO mouse (a double gene deletion of the leptin receptor and PPARγ) and a minor decrease in white adipose tissue (WAT) from ob/ob mice. In skeletal muscle TAG(48:2) and TAG(48:1) were decreased in the POKO mouse but not in ob/ob. All of these mentioned TAGs were also found to be altered in our study, demonstrating that the triglyceride pool is selectively modulated by obesity, type 2 diabetes and dietary intervention.
Another study, where subjects with metabolic syndrome were subjected to a 33-week diet intervention study, has shown a similar rearrangement of chain length in plasma TAGs (Schwab et al. 2008). The authors demonstrate a decrease in short chain saturated fatty acids and that this change is correlated to insulin sensitivity. Despite the low sample size this study did benefit from a wider lipidomic coverage, probably rendering the analysis more sensitive to lipid remodelling. However our study showed clearly that weight loss with the addition of dietary fish oil supplementation causes a large rearrangement of the lipid triglyceride pool.
The complimentary nature of a combined GC-MS and LC-MS approach was evident from the cross correlation of the two data sets. For example, the decrease in the fatty acids 18:3 and 20:3 with fish oil supplementation as determined by GC-MS is consistent with the decrease of PC(36:3) and PC(38:3) if one assumes that one of the fatty acids in these species is the highly abundant palmitate (16:0—Fig. 3b). Neither of these fatty acids correlates with any TAG reduction indicating that the decrease in 18:3 and 20:3 fatty acids preferentially affect PC lipids rather than triglycerides. This suggests that LC n-3 PUFA increase the metabolism of short chain n-3 PC.
As shown by the LC-MS data, the wide variety of lipid types (phospholipids and TAG/DAG) that increase with fish oil supplementation all have a high degree of unsaturation. This is most likely due to the effect of lipid synthesis with the supplemented n-3 PUFA. Unfortunately, one complication in interpreting the results produced in this study is that it was not possible to discriminate between effects associated with fish oil supplementation alone and the combined action of fish oil and weight loss as the study design did not include a group supplemented with fish oil in the absence of weight loss (Krebs et al. 2006). The inclusion of this group would have allowed separation of the weight loss and fish oil effects. Hence, it remains unclear as to which of the changes are due to the fish oil intake alone and which of them may be due to a synergistic weight loss/fish oil supplementation effect.
One of the lipids with a relatively high degree of saturation which increased in concentration in the fish oil supplemented group was SM(38:2). Conversely the concentrations of SM(42:1) and SM(42:2) have been found to decrease in ob/ob mouse liver (Yetukuri 2007). Thus, this increase in SM may be a beneficial effect of fish oil feeding although the exact mechanism remains to be elucidated underlying the complexity of the data set obtained by LC-MS lipidomics.
The changes observed based on weight loss alone include the increase in concentration of SM(30:1) containing very short chained fatty acids. In addition a decrease in TAG(52:1) was detected. This might suggest a decrease in specific TAGs rather than the general reduction of short chain species we observe with the fish oil supplemented groups. However, these were some of the few biological changes associated with weight loss and placebo oil detected across this study, in part because of the study design and the very large changes associated with dietary intervention which dominate the dataset.
The chromatogram peak representing fatty acid 22:5 (a sum of n-6 and n-3 fatty acids) displayed an interesting behaviour as determined by GC-MS. Despite it being the third most abundant fatty acid in the fish oil supplementation (Fig. 3a) it decreased in the comparison between BFO and IFO (Fig. 3c, d) after fish oil supplementation. However, this fatty acid decreased in the weight loss group at 12 and 24 weeks with higher significance as determined by the relative intensity of the loadings. This leads to the conclusion, that weight loss decreases 22:5 but that this reduction is masked slightly by the supplement of fish oil in the diet, so that although weight loss results in an overall decrease between the BFO and IFO group this is not as large as that detected between BPO and IPO. The decrease in the fatty acid is also reflected in the decrease of serum TAG(54:5) which might well be made up of 2 16:0 (one of the most common fatty acids—Fig. 3b) and one 22:5 fatty acids. TAG(54:5) therefore is an exception of a decreasing serum TAG with more than 52 carbons. Interestingly, TAG(54:5) has been reported to decrease in WAT in ob/ob mice and increase in the double knock out POKO in the same tissue as compared to wild type (Medina-Gomez 2007). Assuming that TAG(54:5) contains fatty acid 22:5 this means there is an inverse correlation of the amount of 22:5 in WAT and serum. 22:5 is synthesised from 18:2. The last step of the synthesis takes place in the peroxisomes (Innis 2003; Innis et al. 2004). The decrease in ob/ob WAT but increase in the POKO mouse suggests that PPARγ promotes the mobilisation of 22:5 from adipose tissue. In respect to the study investigated here this suggests that weight loss increases the PPARγ activity in WAT cells.
This result also illustrates the difficulty of ultimately identifying the exact lipid composition in the samples. This holds particularly true for low abundance lipid species. The number of structural isomers within lipids is enormous and the reliable separation and quantification of all the lipid species is in the centre of active, ongoing research. With time this will lead to more assertive assignments and ultimately improved biological interpretation of disease processes in the future.
Surprisingly there were also detectable differences between groups that were planned to be similar. For instance the two baseline groups BPO and BFO (Fig. 6) have a relatively high Q2 of almost 0.4. This indicates the difficulty of randomising people even with ~30 subjects in each group. Furthermore, such studies are also affected by human behaviour. Being part of a study as a control group is most likely going to influence the behaviour of the control group subjects. Indeed we would expect this effect to be most pronounced before samples are taken. This suggests that blood plasma based studies should perhaps be considered alongside material with a slower changing profile such as red blood cells or adipose tissue.
This study investigated fish oil supplemented diet in combination with weight loss by a variety of metabolomic methods. The fish oil supplementation was found to have the largest effect on plasma metabolic profiles. Furthermore only the lipid fraction seemed to be affected as was determined by high resolution 1H-NMR spectroscopy. Fish oil supplementation in conjunction with weight loss was found to reduce the total serum TAG content by selectively reducing the short chain fatty acids. Weight loss caused a reduction in the fatty acid 22:5, although this fatty acid was also increased by fish oil supplementation relative to individuals receiving placebo oil. Overall, these results demonstrate the selective modulation of blood plasma TAGs by fish oil supplementation.
This work was supported by the United Kingdom NIHR Cambridge Biomedical Research Centre. In addition GM and JLG were supported by the British Heart Foundation, the Medical Research Council and the Royal Society, UK. JLG & SAJ are members of the Medical Research Council Centre for Obesity and Related Metabolic Diseases (MRC CORD), and an affiliate member of the University of Cambridge Metabolic Research Laboratories. The authors would like to thank Dr. Richard Knochenmuss for the use of his in-house spectral alignment tool for IGOR, Dr Jeremy Krebs for the original study design and sample collection, and the Nutritional Biochemistry Laboratory at MRC Human Nutrition Research for GC-MS analysis.