, Volume 5, Issue 3, pp 307-317
Date: 03 Feb 2009

Quantitative analysis of metabolite concentrations in human urine samples using 13C{1H} NMR spectroscopy

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Abstract

Targeted profiling is a library-based method of using mathematically modeled reference spectra for quantification of metabolite concentrations in NMR mixture analysis. Metabolomics studies of biofluids, such as urine, represent a highly complex problem in this area, and for this reason targeted profiling of 1H NMR spectra can be hampered. A number of the issues relating to 1H NMR spectroscopy can be overcome using 13C{1H} NMR spectroscopy. In this work, a 13C{1H} NMR database was created using Chenomx NMR Suite, incorporating 120 metabolites. The 13C{1H} NMR database was standardized through the analysis of a series of metabolite solutions containing varying concentrations of 19 distinct metabolites, where the metabolite concentrations were varied across a range of values including biological ranges. Subsequently, the NMR spectra of urine samples were collected using 13C{1H} NMR spectroscopy and profiled using the 13C{1H} NMR library. In total, about 30 metabolites were conclusively identified and quantified in the urine samples using 13C{1H} NMR targeted profiling. The proton decoupling and larger spectral window provided easier identification and more accurate quantification for specific classes of metabolites, such as sugars and amino acids with overlap in the aliphatic region of the 1H NMR spectrum. We discuss potential application areas in which 13C{1H} NMR targeted profiling may be superior to 1H NMR targeted profiling.