Original Article

Metabolomics

, Volume 5, Issue 2, pp 183-198

Proton NMR quantitative profiling for quality assessment of greenhouse-grown tomato fruit

  • Catherine DebordeAffiliated withINRA, UMR619 Biologie du Fruit, IFR103 BVIPlateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI Email author 
  • , Mickaël MaucourtAffiliated withPlateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVIUniversité de Bordeaux, UMR619 Biologie du Fruit
  • , Pierre BaldetAffiliated withINRA, UMR619 Biologie du Fruit, IFR103 BVI
  • , Stéphane BernillonAffiliated withINRA, UMR619 Biologie du Fruit, IFR103 BVIPlateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI
  • , Benoît BiaisAffiliated withINRA, UMR619 Biologie du Fruit, IFR103 BVIPlateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI
  • , Gilles TalonAffiliated withHortis Aquitaine
  • , Carine FerrandAffiliated withDépartement Universitaire des Sciences d’Agen, Université de Bordeaux
  • , Daniel JacobAffiliated withUniversité de Bordeaux, Centre de Bioinformatique de Bordeaux – Génomique Fonctionnelle BordeauxINRA, UMA1251, IFR103 BVI
  • , Hélène Ferry-DumazetAffiliated withUniversité de Bordeaux, Centre de Bioinformatique de Bordeaux – Génomique Fonctionnelle Bordeaux
    • , Antoine de DaruvarAffiliated withUniversité de Bordeaux, Centre de Bioinformatique de Bordeaux – Génomique Fonctionnelle BordeauxUniversité de Bordeaux, UMR 5800 Laboratoire Bordelais de Recherche en InformatiqueCNRS, UMR 5800 Laboratoire Bordelais de Recherche en Informatique
    • , Dominique RolinAffiliated withUniversité de Bordeaux, UMR619 Biologie du Fruit
    • , Annick MoingAffiliated withINRA, UMR619 Biologie du Fruit, IFR103 BVI

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Abstract

Tomato is an essential crop in terms of economic importance and nutritional quality. In France, the third most important region for tomato (Solanum lycopersicum L.) production is Aquitaine where the major part of production is now grown soilless under greenhouse conditions with harvest from March to November. Tomato fruit quality at harvest is a direct function of its metabolite content at that time. The aim of this work was to use a global approach to characterize changes in the fruit organoleptic quality at harvest under commercial culture conditions during an entire season for two varieties and two different fertilization practices (with or without recycling of the nutrient solution) for one variety. Absolute quantification data of 32 major compounds in fruit without seeds were obtained through untargeted (proton nuclear magnetic resonance, 1H-NMR) quantitative profiling. These data were complemented by colorimetric analysis of ascorbate and total phenolics. They were analyzed with chemometric approaches. Principal component analysis (PCA) or partial least square analyses (PLS) revealed more discriminant metabolites for season than for variety and showed that nutrient solution recycling had very little effect on fruit composition. These tendencies were confirmed with univariate analyses. 1H-NMR profiling complemented with colorimetric analyses therefore provided a diagnostic tool to follow the changes in organoleptic and nutritional quality of tomato. In addition the quantitative information generated will help to increase our knowledge on the mechanisms of plant response to environmental modifications.

Keywords

Solanum lycopersicum Fruit quality 1H-NMR Polar metabolites Metabolic fingerprinting Metabolomics Cultivation practices