Metabolomics

, Volume 5, Issue 2, pp 183–198

Proton NMR quantitative profiling for quality assessment of greenhouse-grown tomato fruit

Authors

    • INRA, UMR619 Biologie du Fruit, IFR103 BVI
    • Plateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI
  • Mickaël Maucourt
    • Plateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI
    • Université de Bordeaux, UMR619 Biologie du Fruit
  • Pierre Baldet
    • INRA, UMR619 Biologie du Fruit, IFR103 BVI
  • Stéphane Bernillon
    • INRA, UMR619 Biologie du Fruit, IFR103 BVI
    • Plateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI
  • Benoît Biais
    • INRA, UMR619 Biologie du Fruit, IFR103 BVI
    • Plateforme Métabolome-Fluxome de Génomique Fonctionnelle Bordeaux, IFR103 BVI
  • Gilles Talon
    • Hortis Aquitaine
  • Carine Ferrand
    • Département Universitaire des Sciences d’AgenUniversité de Bordeaux
  • Daniel Jacob
    • Université de Bordeaux, Centre de Bioinformatique de Bordeaux – Génomique Fonctionnelle Bordeaux
    • INRA, UMA1251, IFR103 BVI
  • Hélène Ferry-Dumazet
    • Université de Bordeaux, Centre de Bioinformatique de Bordeaux – Génomique Fonctionnelle Bordeaux
  • Antoine de Daruvar
    • Université de Bordeaux, Centre de Bioinformatique de Bordeaux – Génomique Fonctionnelle Bordeaux
    • Université de Bordeaux, UMR 5800 Laboratoire Bordelais de Recherche en Informatique
    • CNRS, UMR 5800 Laboratoire Bordelais de Recherche en Informatique
  • Dominique Rolin
    • Université de Bordeaux, UMR619 Biologie du Fruit
  • Annick Moing
    • INRA, UMR619 Biologie du Fruit, IFR103 BVI
Original Article

DOI: 10.1007/s11306-008-0134-2

Cite this article as:
Deborde, C., Maucourt, M., Baldet, P. et al. Metabolomics (2009) 5: 183. doi:10.1007/s11306-008-0134-2

Abstract

Tomato is an essential crop in terms of economic importance and nutritional quality. In France, the third most important region for tomato (Solanum lycopersicum L.) production is Aquitaine where the major part of production is now grown soilless under greenhouse conditions with harvest from March to November. Tomato fruit quality at harvest is a direct function of its metabolite content at that time. The aim of this work was to use a global approach to characterize changes in the fruit organoleptic quality at harvest under commercial culture conditions during an entire season for two varieties and two different fertilization practices (with or without recycling of the nutrient solution) for one variety. Absolute quantification data of 32 major compounds in fruit without seeds were obtained through untargeted (proton nuclear magnetic resonance, 1H-NMR) quantitative profiling. These data were complemented by colorimetric analysis of ascorbate and total phenolics. They were analyzed with chemometric approaches. Principal component analysis (PCA) or partial least square analyses (PLS) revealed more discriminant metabolites for season than for variety and showed that nutrient solution recycling had very little effect on fruit composition. These tendencies were confirmed with univariate analyses. 1H-NMR profiling complemented with colorimetric analyses therefore provided a diagnostic tool to follow the changes in organoleptic and nutritional quality of tomato. In addition the quantitative information generated will help to increase our knowledge on the mechanisms of plant response to environmental modifications.

Keywords

Solanum lycopersicumFruit quality1H-NMRPolar metabolitesMetabolic fingerprintingMetabolomicsCultivation practices

Abbreviations

AEAC

Ascorbic acid equivalent antioxidant capacity

DW

Dry weight

EC

Electroconductivity

HCA

Hierarchical clustering analysis

NMR

Nuclear magnetic resonance

PCA

Principal component analysis

PLS

Partial least square regression

TSP

(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC 2008