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Polyploidy, B chromosomes, and heterochromatin characterization of Mimosa caesalpiniifolia Benth. (Fabaceae-Mimosoideae)

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Abstract

Eight populations of Mimosa caesalpiniifolia Benth. were investigated using a cytogenetic approach. Here, we describe for the first time details of the karyotype including chromosome morphology, physical mapping of chromomycin A3 (CMA) 4′,6-diamidino-2-phenylindole (DAPI) and silver staining of nucleolar organizer regions (Ag-NOR banding), as well as 45S rDNA sites. All populations studied showed karyotypes with 2n = 2x = 26 small metacentric and submetacentric chromosomes, although some individuals exhibited 2n = 4x = 52 chromosomes. Moreover, we observed putative additional B chromosomes in some populations. The CMA banding and fluorescent in situ hybridization techniques revealed NOR heteromorphism on the unique pair containing 45 rDNA site (chromosome 12) while the Ag-NOR banding indicated NORs on both cytotypes. Up to two and four nucleoli were observed, respectively, on individuals with 2n = 2x = 26 and 2n = 4x = 52 chromosomes and the differences in nucleolar size seems to be directly related to NOR heteromorphism in some individuals. The data present new and important information to understand karyotypic evolution of Mimosa and Fabaceae.

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Acknowledgments

The authors thank Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, and Conselho Nacional de Desenvolvimento Científico e Tecnológico for financial support.

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Authors and Affiliations

  1. Genetics Laboratory, Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, Minas Gerais, Brazil

    Saulo Marçal de Sousa, Aryane Campos Reis & Lyderson Facio Viccini

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  1. Saulo Marçal de Sousa
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  2. Aryane Campos Reis
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  3. Lyderson Facio Viccini
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Correspondence to Saulo Marçal de Sousa.

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Communicated by R. Sederoff

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de Sousa, S.M., Reis, A.C. & Viccini, L.F. Polyploidy, B chromosomes, and heterochromatin characterization of Mimosa caesalpiniifolia Benth. (Fabaceae-Mimosoideae). Tree Genetics & Genomes 9, 613–619 (2013). https://doi.org/10.1007/s11295-012-0567-7

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