Tree Genetics & Genomes

, Volume 7, Issue 1, pp 63–77

Genotyping systems for Eucalyptus based on tetra-, penta-, and hexanucleotide repeat EST microsatellites and their use for individual fingerprinting and assignment tests

Authors

  • Danielle Assis Faria
    • Plant Genetics LaboratoryEmbrapa–Recursos Genéticos e Biotecnologia
    • Graduate Program in Genomic Sciences and BiotechnologyUniversidade Católica de Brasília–SGAN 916 modulo B
  • Eva Maria Celia Mamani
    • Plant Genetics LaboratoryEmbrapa–Recursos Genéticos e Biotecnologia
    • Department of Cell BiologyUniversidade de Brasília–UnB
  • Georgios Joannis PappasJr.
    • Plant Genetics LaboratoryEmbrapa–Recursos Genéticos e Biotecnologia
    • Graduate Program in Genomic Sciences and BiotechnologyUniversidade Católica de Brasília–SGAN 916 modulo B
    • Plant Genetics LaboratoryEmbrapa–Recursos Genéticos e Biotecnologia
    • Department of Cell BiologyUniversidade de Brasília–UnB
    • Graduate Program in Genomic Sciences and BiotechnologyUniversidade Católica de Brasília–SGAN 916 modulo B
Original Paper

DOI: 10.1007/s11295-010-0315-9

Cite this article as:
Faria, D.A., Mamani, E.M.C., Pappas, G.J. et al. Tree Genetics & Genomes (2011) 7: 63. doi:10.1007/s11295-010-0315-9

Abstract

Eucalypts are keystone species in their natural ranges and are extensively planted worldwide for high-quality woody biomass. A novel set of 21 polymorphic and interspecifically transferable microsatellite markers based on tetra-, penta- and hexanucleotide repeats were developed and tested for high-precision genotyping of species of Eucalyptus. These microsatellites were characterized in population samples of four species, Eucalyptus grandis, Eucalyptus globulus, Eucalyptus urophylla, and Eucalyptus camaldulensis, representing three phylogenetic sections of subgenus Symphyomyrtus. These markers provide a clear advantage for accurate allele calling due to their larger allele size difference. Two multiplexed microsatellite combinations, a 14-locus/four-dye and an 18-locus/five-dye set, analyzable in single lanes were designed, providing resolution and throughput analogous to those routinely used in human DNA profiling. This set of microsatellites was shown to have high resolution for clone fingerprinting, inter-individual genetic distance estimation, species distinction, and assignment of hybrid individuals to their most likely ancestral species. These systems will be particularly useful for comparative population genetics and molecular breeding applications that require consistent allele calling across different points in time or laboratories.

Keywords

MicrosatellitesMultiplexAssignment testsEucalyptus

Supplementary material

11295_2010_315_MOESM1_ESM.pdf (69 kb)
Supplementary Figure S1(PDF 69 kb)
11295_2010_315_MOESM2_ESM.pdf (103 kb)
Supplementary Table S1(PDF 103 kb)

Copyright information

© Springer-Verlag 2010