Tree Genetics & Genomes

, Volume 6, Issue 5, pp 689–700

Prunus microsatellite marker transferability across rosaceous crops

  • Mourad Mnejja
  • Jordi Garcia-Mas
  • Jean-Marc Audergon
  • Pere Arús
Original Paper

DOI: 10.1007/s11295-010-0284-z

Cite this article as:
Mnejja, M., Garcia-Mas, J., Audergon, JM. et al. Tree Genetics & Genomes (2010) 6: 689. doi:10.1007/s11295-010-0284-z

Abstract

A total of 145 microsatellite primer pairs from Prunus DNA sequences were studied for transferability in a set of eight cultivars from nine rosaceous species (almond, peach, apricot, Japanese plum, European plum, cherry, apple, pear, and strawberry), 25 each of almond genomic, peach genomic, peach expressed sequence tags (EST), and Japanese plum genomic, 22 of almond EST, and 23 of apricot (13 EST and 10 genomic), all known to produce single-locus and polymorphic simple-sequence repeats in the species where they were developed. Most primer pairs (83.6%) amplified bands of the expected size range in other Prunus. Transferability, i.e., the proportion of microsatellites that amplified and were polymorphic, was also high in Prunus (63.9%). Almond and Japanese plum were the most variable among the diploid species (all but the hexaploid European plum) and peach the least polymorphic. Thirty-one microsatellites amplified and were polymorphic in all Prunus species studied, 12 of which, covering its whole genome, are proposed as the “universal Prunus set”. In contrast, only 16.3% were transferable in species of other Rosaceae genera (apple, pear, and strawberry). Polymorphic Prunus microsatellites also detected lower levels of variability in the non-congeneric species. No significant differences were detected in transferability and the ability to detect variability between microsatellites of EST and genomic origin.

Keywords

TransferabilityRosaceaeUniversal Prunus SSR set

Supplementary material

11295_2010_284_MOESM1_ESM.doc (62 kb)
Table 1SEST-derived almond microsatellites (DOC 62 kb)
11295_2010_284_MOESM2_ESM.doc (63 kb)
Table 2SEST-derived peach microsatellites (DOC 63 kb)
11295_2010_284_MOESM3_ESM.doc (812 kb)
Table 3SVariability of 145 Prunus SSRs in nine rosaceous species (DOC 811 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Mourad Mnejja
    • 1
  • Jordi Garcia-Mas
    • 1
  • Jean-Marc Audergon
    • 2
  • Pere Arús
    • 1
  1. 1.IRTACentre de Recerca en Agrigenòmica CSIC–IRTA–UAB08348Cabrils (Barcelona)Spain
  2. 2.INRAUnité de Génétique et Amélioration des Fruits et LégumesMontfavetFrance