Tree Genetics & Genomes

, Volume 5, Issue 1, pp 81–91

Expression profiling in HcrVf2-transformed apple plants in response to Venturia inaequalis

  • Roberta Paris
  • Valentina Cova
  • Giulia Pagliarani
  • Stefano Tartarini
  • Matteo Komjanc
  • Silviero Sansavini
Original Paper

DOI: 10.1007/s11295-008-0177-6

Cite this article as:
Paris, R., Cova, V., Pagliarani, G. et al. Tree Genetics & Genomes (2009) 5: 81. doi:10.1007/s11295-008-0177-6

Abstract

Apple scab resistance is one of the most well-characterized plant–pathogen interactions in a woody plant species. While the HcrVf2 gene from the wild apple Malus floribunda 821 has proved capable of conferring scab resistance to the susceptible cv. Gala after genetic transformation, its identification represents only the first step in understanding the molecular mechanisms and, hence, the network of genes underlying the defence response. We used a PCR-based suppression subtractive hybridization to identify apple genes that are differentially expressed after Venturia inaequalis inoculation. Subtractive hybridization was performed between cDNA from challenged leaves of HcrVf2-resistant transgenic Gala and susceptible cv. Gala plants. A library of 523 unigenes was constructed and characterized by assigning a putative function via comparison with public databases. This set of pathogen-modulated apple genes includes many defence-related genes and is therefore an important source of information for understanding the molecular basis of the MalusV. inaequalis interaction.

Keywords

Apple scabDefenceSubtracted libraryMalus × domestica

Supplementary material

11295_2008_177_MOESM1_ESM.doc (550 kb)
ESM 1List of the 558 single-pass sequences of the apple SSH library (DOC 602 KB)
11295_2008_177_MOESM2_ESM.xls (104 kb)
Table S1List of the 334 unigenes identified by SSH and similar to known genes available in public databases. 264 clones were derived from the ‘forward’ subtraction, 59 from the ‘reverse’ and 11 from both the procedures (XLS 103 KB)
11295_2008_177_MOESM3_ESM.xls (58 kb)
Table S2List of ESTs that (1) did not match any sequence in the database (NO HIT), (2) match sequences of unknown function (UNCLASSIFIED); (3) match sequences of unclear function (UNCLEAR FUNCTION); (4) match transposon-related sequences (TRANSPOSONS) (XLS 58.0 KB)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Roberta Paris
    • 1
  • Valentina Cova
    • 1
    • 2
  • Giulia Pagliarani
    • 1
  • Stefano Tartarini
    • 1
  • Matteo Komjanc
    • 2
  • Silviero Sansavini
    • 1
  1. 1.Department of Fruit Tree and Woody Plant SciencesUniversity of BolognaBolognaItaly
  2. 2.Genetics and Molecular Biology DepartmentIASMA Research CentreMichele all’Adige (TN)Italy