Tree Genetics & Genomes

, Volume 4, Issue 4, pp 745–756

A framework physical map for peach, a model Rosaceae species

Authors

    • Department of Genetics and BiochemistryClemson University
  • G. Swire-Clark
    • Department of HorticultureClemson University
  • L. L. Georgi
    • Department of Genetics and BiochemistryClemson University
  • L. Garay
    • Department of Genetics and BiochemistryClemson University
  • S. Jung
    • Department of Horticulture and Landscape ArchitectureWashington State University
  • S. Forrest
    • Department of Genetics and BiochemistryClemson University
  • A. V. Blenda
    • Department of Genetics and BiochemistryClemson University
  • B. Blackmon
    • Clemson University Genomics InstituteClemson University
  • J. Mook
    • Department of Genetics and BiochemistryClemson University
    • Agricultural Research ProgramsVirginia State University
  • R. Horn
    • Department of Genetics and BiochemistryClemson University
    • Abt Pflanzengenetik, Institut für BiowissenschaftenUniversität Rostock
  • W. Howad
    • Centre de Recerca en Agrigenòmica CSIC–IRTA–UAB
  • P. Arús
    • Centre de Recerca en Agrigenòmica CSIC–IRTA–UAB
  • D. Main
    • Department of Horticulture and Landscape ArchitectureWashington State University
  • J. P. Tomkins
    • Clemson University Genomics InstituteClemson University
  • B. Sosinski
    • Department of Horticultural SciencesNorth Carolina State University
  • W. V. Baird
    • Department of HorticultureClemson University
  • G. L. Reighard
    • Department of HorticultureClemson University
  • A. G. Abbott
    • Department of Genetics and BiochemistryClemson University
Original Paper

DOI: 10.1007/s11295-008-0147-z

Cite this article as:
Zhebentyayeva, T.N., Swire-Clark, G., Georgi, L.L. et al. Tree Genetics & Genomes (2008) 4: 745. doi:10.1007/s11295-008-0147-z

Abstract

A genome-wide framework physical map of peach was constructed using high-information content fingerprinting (HICF) and FPC software. The resulting HICF assembly contained 2,138 contigs composed of 15,655 clones (4.3× peach genome equivalents) from two complementary bacterial artificial chromosome libraries. The total physical length of all contigs is estimated at 303 Mb or 104.5% of the peach genome. The framework physical map is anchored on the Prunus genetic reference map and integrated with the peach transcriptome map. The physical length of anchored contigs is estimated at 45.0 Mb or 15.5% of the genome. Altogether, 2,636 markers, i.e., genetic markers, peach unigene expressed sequence tags, and gene-specific and overgo probes, were incorporated into the physical framework and supported the accuracy of contig assembly.

Keywords

Prunus persicaHICF fingerprintingBAC-based physical/genetic map

Supplementary material

11295_2008_147_MOESM1_ESM.doc (223 kb)
Table 1List of genetic markers integrated into peach HICF physical map (DOC 223 KB).

Copyright information

© Springer-Verlag 2008