Tree Genetics & Genomes

, Volume 4, Issue 4, pp 727–743

Construction of an integrated consensus map of the apple genome based on four mapping populations

  • A. N’Diaye
  • W. E. Van de Weg
  • L. P. Kodde
  • B. Koller
  • F. Dunemann
  • M. Thiermann
  • S. Tartarini
  • F. Gennari
  • C. E. Durel
Original Paper

DOI: 10.1007/s11295-008-0146-0

Cite this article as:
N’Diaye, A., Van de Weg, W.E., Kodde, L.P. et al. Tree Genetics & Genomes (2008) 4: 727. doi:10.1007/s11295-008-0146-0

Abstract

An integrated consensus genetic map for apple was constructed on the basis of segregation data from four genetically connected crosses (C1 = Discovery × TN10-8, C2 = Fiesta × Discovery, C3 = Discovery × Prima, C4 = Durello di Forli × Fiesta) with a total of 676 individuals using CarthaGene® software. First, integrated female–male maps were built for each population using common female–male simple sequence repeat markers (SSRs). Then, common SSRs over populations were used for the consensus map integration. The integrated consensus map consists of 1,046 markers, of which 159 are SSR markers, distributed over 17 linkage groups reflecting the basic chromosome number of apple. The total length of the integrated consensus map was 1,032 cM with a mean distance between adjacent loci of 1.1 cM. Markers were proportionally distributed over the 17 linkage groups (χ2 = 16.53, df = 16, p = 0.41). A non-uniform marker distribution was observed within all of the linkage groups (LGs). Clustering of markers at the same position (within a 1-cM window) was observed throughout LGs and consisted predominantly of only two to three linked markers. The four integrated female–male maps showed a very good colinearity in marker order for their common markers, except for only two (CH01h01, CH05g03) and three (CH05a02z, NZ02b01, Lap-1) markers on LG17 and LG15, respectively. This integrated consensus map provides a framework for performing quantitative trait locus (QTL) detection in a multi-population design and evaluating the genetic background effect on QTL expression.

Keywords

Malus × domestica Borkh.Integrated consensus genetic mapFemale–male mapSSR marker

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • A. N’Diaye
    • 1
  • W. E. Van de Weg
    • 2
  • L. P. Kodde
    • 2
  • B. Koller
    • 3
  • F. Dunemann
    • 4
  • M. Thiermann
    • 4
  • S. Tartarini
    • 5
  • F. Gennari
    • 5
  • C. E. Durel
    • 1
  1. 1.UMR1259 Génétique et Horticulture (GenHort), INRABeaucouzéFrance
  2. 2.Department of Plant BreedingWageningen University and Research Centre, Plant Research InternationalWageningenThe Netherlands
  3. 3.Ecogenics GmbHZurich-SchlierenSwitzerland
  4. 4.Bundesanstalt für Züchtungsforschung an Kulturpflanzen, Institut für ObstzüchtungDresdenGermany
  5. 5.Department of Fruit Tree and Woody Plant ScienceUniversity of BolognaBolognaItaly